Cryo-EM analysis of the HCoV-229E spike glycoprotein reveals dynamic prefusion conformational changes

Xiyong Song; Yuejun Shi; Wei Ding; Tongxin Niu; Limeng Sun; Yubei Tan; Yong Chen; Jiale Shi; Qiqi Xiong; Xiaojun Huang; Shaobo Xiao; Yanping Zhu; Chongyun Cheng; Zhen F. Fu; Zhi-Jie Liu; Guiqing Peng

doi:10.1038/s41467-020-20401-y

Nature Communications (Jan 2021)

Cryo-EM analysis of the HCoV-229E spike glycoprotein reveals dynamic prefusion conformational changes

Xiyong Song,
Yuejun Shi,
Wei Ding,
Tongxin Niu,
Limeng Sun,
Yubei Tan,
Yong Chen,
Jiale Shi,
Qiqi Xiong,
Xiaojun Huang,
Shaobo Xiao,
Yanping Zhu,
Chongyun Cheng,
Zhen F. Fu,
Zhi-Jie Liu,
Guiqing Peng

Affiliations

Xiyong Song: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University
Yuejun Shi: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University
Wei Ding: CAS Key Laboratory of Soft Matter Physics, Institute of Physics, Chinese Academy of Sciences
Tongxin Niu: Center for Biological Imaging, Institute of Biophysics, Chinese Academy of Sciences
Limeng Sun: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University
Yubei Tan: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University
Yong Chen: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences
Jiale Shi: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University
Qiqi Xiong: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University
Xiaojun Huang: Center for Biological Imaging, Institute of Biophysics, Chinese Academy of Sciences
Shaobo Xiao: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University
Yanping Zhu: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences
Chongyun Cheng: National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences
Zhen F. Fu: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University
Zhi-Jie Liu: Institute of Molecular and Clinical Medicine, Kunming Medical University
Guiqing Peng: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University

DOI: https://doi.org/10.1038/s41467-020-20401-y
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 9

Abstract

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The spike protein of coronaviruses (S-protein) is an envelope-anchored trimeric type I transmembrane glycoprotein that mediates receptor binding and the fusion of the viral and host cell membranes. Here the authors report the conformational states of HCoV-229E S trimer and observe the conformational changes in S1 subunit from closed state to activated state for receptor binding.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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