Analysis of the Antioxidant Mechanism of Ozone Treatment to Extend the Shelf Life and Storage Quality of ‘Korla’ Fragrant Pears Based on Label-Free Proteomics
Shaohua Lin,
Xiaojun Zhang,
Mo Li,
Na Zhang,
Chenghu Dong,
Haipeng Ji,
Pufan Zheng,
Zhaojun Ban,
Xing Mei,
Changyu Gu,
Cunkun Chen
Affiliations
Shaohua Lin
Beijing Vocational College of Agriculture, Beijing 102442, China
Xiaojun Zhang
School of Basic Medical Sciences, Future Health Laboratory of Innovation Center of Yangtze River Delta, Zhejiang University, Hangzhou 314100, China
Mo Li
School of Agriculture and Environment, Massey University, Palmerston North 4410, New Zealand
Na Zhang
National Engineering Technology Research Center for Preservation of Agriculture Product, Institute of Agricultural Products Preservation and Processing Technology, Tianjin Academy of Agricultural Sciences, Tianjin 300384, China
Chenghu Dong
National Engineering Technology Research Center for Preservation of Agriculture Product, Institute of Agricultural Products Preservation and Processing Technology, Tianjin Academy of Agricultural Sciences, Tianjin 300384, China
Haipeng Ji
National Engineering Technology Research Center for Preservation of Agriculture Product, Institute of Agricultural Products Preservation and Processing Technology, Tianjin Academy of Agricultural Sciences, Tianjin 300384, China
Pufan Zheng
National Engineering Technology Research Center for Preservation of Agriculture Product, Institute of Agricultural Products Preservation and Processing Technology, Tianjin Academy of Agricultural Sciences, Tianjin 300384, China
Zhaojun Ban
Zhejiang Provincial Key Laboratory of Chemical and Biological Processing Technology of Farm Products, School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China
Xing Mei
College of Continuing Education, Wuhan Polytechnic University, Wuhan 430023, China
Changyu Gu
Sinolight Inspection & Certification Co., Ltd., Beijing 102442, China
Cunkun Chen
National Engineering Technology Research Center for Preservation of Agriculture Product, Institute of Agricultural Products Preservation and Processing Technology, Tianjin Academy of Agricultural Sciences, Tianjin 300384, China
In this study, the physiological indicators, reactive oxygen species (ROS) levels, and activities and expressions of key enzymes related to ROS metabolism were monitored to explore the mechanism of ozone treatment on the shelf life of postharvest Korla fragrant pears. The results show that postharvest fragrant pears treated with ozone had a higher firmness and lower weight loss rate and decay rate during their shelf life, especially in the late stage. Ozone treatment could also delay the occurrence of the respiratory peak and reduce the peak value. The generation rate of superoxide anion (O2−), the hydrogen peroxide (H2O2) content, and the malondialdehyde (MDA) level were reduced in the ozone-treated group, while the activities of key enzymes, superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD), related to ROS metabolism in postharvest fragrant pears were stimulated by ozone treatment, especially in the middle and late stages of shelf life. Analysis of the proteomics results indicates that the POD family enzymes were the main target protein in postharvest fragrant pears treated by ozone during the middle and late stages of shelf life. The activity and expression of antioxidant-related enzymes in postharvest fragrant pears were stimulated by ozone to accelerate the metabolism of ROS and maintain high quality, especially in the middle and late shelf lives.
