Nature Communications (Nov 2017)

High-intensity UV laser ChIP-seq for the study of protein-DNA interactions in living cells

  • Arndt Steube,
  • Tino Schenk,
  • Alexander Tretyakov,
  • Hans Peter Saluz

DOI
https://doi.org/10.1038/s41467-017-01251-7
Journal volume & issue
Vol. 8, no. 1
pp. 1 – 9

Abstract

Read online

Chromatin immunoprecipitation followed by DNA sequencing (ChIP-seq) can map transcription factor binding to a given genome. Here, Steube and colleagues devise a ChIP-seq technique based on photochemical crosslinking by high-intensity ultraviolet laser irradiation, and describe its utility.