Nature Communications (Nov 2017)
High-intensity UV laser ChIP-seq for the study of protein-DNA interactions in living cells
Abstract
Chromatin immunoprecipitation followed by DNA sequencing (ChIP-seq) can map transcription factor binding to a given genome. Here, Steube and colleagues devise a ChIP-seq technique based on photochemical crosslinking by high-intensity ultraviolet laser irradiation, and describe its utility.