PLoS ONE (Jan 2018)

Effects of a single transient transfection of Ten-eleven translocation 1 catalytic domain on hepatocellular carcinoma.

  • Yuying Liu,
  • Hui Zhu,
  • Zhenxue Zhang,
  • Changchun Tu,
  • Dongyuan Yao,
  • Bin Wen,
  • Ru Jiang,
  • Xing Li,
  • Pengfei Yi,
  • Jiejie Zhan,
  • Jiaping Hu,
  • Jianwu Ding,
  • Liping Jiang,
  • Fanglin Zhang

DOI
https://doi.org/10.1371/journal.pone.0207139
Journal volume & issue
Vol. 13, no. 12
p. e0207139

Abstract

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Tumor suppressor genes (TSGs), including Ten-eleven translocation 1 (TET1), are hypermethylated in hepatocellular carcinoma (HCC). TET1 catalytic domain (TET1-CD) induces genome-wide DNA demethylation to activate TSGs, but so far, anticancer effects of TET1-CD are unclear. Here we showed that after HCC cells were transiently transfected with TET1-CD, the methylation levels of TSGs, namely APC, p16, RASSF1A, SOCS1 and TET1, were distinctly reduced, and their mRNA levels were significantly increased and HCC cells proliferation, migration and invasion were suppressed, but the methylation and mRNA levels of oncogenes, namely C-myc, Bmi1, EMS1, Kpna2 and c-fos, were not significantly change. Strikingly, HCC subcutaneous xenografts in nude mice remained to be significantly repressed even 54 days after transient transfection of TET1-CD. So, transient transfection of TET1-CD may be a great advance in HCC treatment due to its activation of multiple TSGs and persistent anticancer effects.