Ubiquitin transfer by a RING E3 ligase occurs from a closed E2~ubiquitin conformation

Abstract

The mechanism of ubiquitin transfer from the ubiquitin-conjugated E2 enzyme (E2~Ub) to the substrate is still under debate. Here, the authors use FRET assays to show that RING E3 ligases transfer ubiquitin to the substrate from a closed E2~Ub conformation.