Bio-Protocol
(May 2018)
Quantifying Podocytes and Parietal Epithelial Cells in Human Urine Using Liquid-based Cytology and WT1 Immunoenzyme Staining
Hiroyuki Ohsaki,
Toru Matsunaga,
Taishi Fujita,
Yasunori Tokuhara,
Shingo Kamoshida,
Tadashi Sofue
Affiliations
Hiroyuki Ohsaki
Department of Medical Biophysics, Kobe University Graduate School of Health Sciences, Kobe, Japan
Toru Matsunaga
Department of Diagnostic Pathology, University Hospital, Faculty of Medicine, Kagawa University, Kagawa, Japan
Taishi Fujita
Department of Clinical Laboratory, Ehime General Health Care Association, Ehime, Japan
Yasunori Tokuhara
Department of Medical Technology, Ehime Prefectural University of Health Sciences, Ehime, Japan
Shingo Kamoshida
Department of Medical Biophysics, Kobe University Graduate School of Health Sciences, Kobe, Japan
Tadashi Sofue
Department of Cardiorenal and Cerebrovascular Medicine, Faculty of Medicine, Kagawa University, Kagawa, Japan
DOI
https://doi.org/10.21769/BioProtoc.2827
Journal volume & issue
Vol. 8,
no. 9
Abstract
Read online
In glomerular disease, podocytes and parietal epithelial cells (PECs) are shed in the urine. Therefore, urinary podocytes and PECs are noninvasive biomarkers of glomerular disease. The purpose of this protocol is to employ immunocytochemistry to detect podocytes and PECs, using the WT1 antibody on liquid-based cytology slides.
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