Eradication of Pseudomonas aeruginosa biofilms and persister cells using an electrochemical scaffold and enhanced antibiotic susceptibility

Abstract

Antibiotics: Enhanced effect with an electrical scaffold Using an electrically conductive fabric to generate hydrogen peroxide could eradicate persistent biofilms in chronically infected wounds. Electrochemical scaffolds (e-scaffolds) are thin networks of conductive material such as carbon fiber used to generate chemical responses in media they are in contact with. Haluk Beyenal and colleagues at Washington State University, USA, investigated the effect of a carbon fabric e-scaffold on cultured biofilms of the bacterium Pseudomonas aeruginosa. The procedure enhanced the susceptibility of this troublesome multidrug-resistant bacterium to the antibiotic tobramycin. Crucially, it eradicated so-called persister cells that can evade antibiotic treatment to reform biofilms in chronic wounds. The research suggests that the effect involves the production of hydroxyl free radicals from hydrogen peroxide and increased permeability of the bacterial cell membranes. The potential of e-scaffolds for treating infected wounds warrants further exploration.