STABILIZING THE GONADOTROPIN ACTIVITY WITH THE USE OF DIFFERENT ORGANIC COMPOUNDS

Abstract

Complex studies of optimum quantitative and qualitative composition of carbohydrates and amino acids necessary for gonadotropines activity stabilization were the aim of our investigations. This was determined by biological active substances - saccharose, L- lysine which stabilizes gonadotropins activity for longer use while preserving activity. As a result of the studies we found out that during 6 weeks of incubation at 40 0 ɋ, the activity of hCG was the highest in the samples with 50 and 75 mg/sm3 of saccharose. Concentration of the hormone was high at 8 week of the incubation in the group of samples with 75 mg/sm3 of saccharose. In the series of hCG samples with 10 mg/sm3 of L - lysine, the activity of hormone was 54% during 8 weeks. Compatible adding of 10 mg/cm3 of L-lysine + 75 mg/sm3 of saccharose provided the best stabilization gonadotropin activity.

Keywords

• chorionic gonadotropin
• stabilization
• saccharose
• L - lysine
• immune-chemiluminescent assay