Changes of intestinal microflora diversity in diarrhea model of KM mice and effects of Psidium guajava L. as the treatment agent for diarrhea

Junjia Lu; Dechang Mao; Xiang Li; Yunqiang Ma; Yunqi Luan; Yong Cao; Yunpeng Luan

Journal of Infection and Public Health (Jan 2020)

Changes of intestinal microflora diversity in diarrhea model of KM mice and effects of Psidium guajava L. as the treatment agent for diarrhea

Junjia Lu,
Dechang Mao,
Xiang Li,
Yunqiang Ma,
Yunqi Luan,
Yong Cao,
Yunpeng Luan

Affiliations

Junjia Lu: College of Biodiversity Conservation and Utilisation, Southwest University, United States
Dechang Mao: Department of Life Technology Teaching and Research, School of Life Science, Southwest Forestry University, PR China
Xiang Li: Department of Immunology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, PR China
Yunqiang Ma: Rearch Institute of Resources and Insects, Chinese Academy of Forestry Chinese Academy of Forest, Kunming, 650224, PR China
Yunqi Luan: Lomonosov Moscow State University, Russia
Yong Cao: Department of Life Technology Teaching and Research, College of Life Sciences, Southwest Forestry University, PR China
Yunpeng Luan: Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, PR China; Department of Life Technology Teaching and Research, School of Life Science, Southwest Forestry University, PR China; Corresponding author at: Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, 300 Bailong Temple, Panlong, Kunming, Yunnan 652400, PR China

Journal volume & issue: Vol. 13, no. 1
pp. 16 – 26

Abstract

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Objectives: This study aimed to explore the effects of Psidium guajava L. extracts on mice diarrhea model and changes in their intestinal microflora diversity. Methods: Mice diarrhea model was constructed with Folium Sennae. and The therapeutic effects of Psidium guajava L. extracts on diarrhea were evaluated by loose stools rates, diarrhea rates, diarrhea index and motor functions of intestine. Results: Our results showed that all three kinds of extracts from Psidium guajava L. had inhibitory effects on Folium Sennae —induced diarrhea model. The proportion of Bacteroidetes is lower in Group DF (diarrhea model group) compared with Group B (blank group), while abundance of Deferribacteraceae was found in Group DF. The proportion of Deferribacteraceae lowered in Group DTF (diarrhea treatment group) treated with extracts from Psidium guajava L. Conclusions: However, mechanisms underlying the therapeutic effect of Psidium guajava L. and the changes in intestinal microflora still await further exploration. Keywords: Psidium guajava L., Diarrhea, Intestinal microflora, ERIC-PCR, 16S-rDNA, Bacteroidetes, Deferribacteraceae

Published in Journal of Infection and Public Health

ISSN: 1876-0341 (Print); 1876-035X (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Medicine: Internal medicine: Infectious and parasitic diseases; Medicine: Public aspects of medicine
Website: https://www.journals.elsevier.com/journal-of-infection-and-public-health

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