Structural characterization of a soil viral auxiliary metabolic gene product – a functional chitosanase

Ruonan Wu; Clyde A. Smith; Garry W. Buchko; Ian K. Blaby; David Paez-Espino; Nikos C. Kyrpides; Yasuo Yoshikuni; Jason E. McDermott; Kirsten S. Hofmockel; John R. Cort; Janet K. Jansson

doi:10.1038/s41467-022-32993-8

Nature Communications (Sep 2022)

Structural characterization of a soil viral auxiliary metabolic gene product – a functional chitosanase

Ruonan Wu,
Clyde A. Smith,
Garry W. Buchko,
Ian K. Blaby,
David Paez-Espino,
Nikos C. Kyrpides,
Yasuo Yoshikuni,
Jason E. McDermott,
Kirsten S. Hofmockel,
John R. Cort,
Janet K. Jansson

Affiliations

Ruonan Wu: Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory
Clyde A. Smith: Stanford Synchrotron Radiation Light source, Stanford University
Garry W. Buchko: Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory
Ian K. Blaby: US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory
David Paez-Espino: Mammoth Biosciences
Nikos C. Kyrpides: US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory
Yasuo Yoshikuni: US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory
Jason E. McDermott: Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory
Kirsten S. Hofmockel: Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory
John R. Cort: Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory
Janet K. Jansson: Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory

DOI: https://doi.org/10.1038/s41467-022-32993-8
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 14

Abstract

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Metagenomics is revealing auxiliary metabolic genes (AMGs) in soil viral genomes. Here, authors solve the crystal structure for a soil viral AMG product, free and ligand bound, and show the protein can decompose chitin, a common carbon polymer.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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