Hyperpolarized [2–13C]pyruvate MR molecular imaging with whole brain coverage
Brian T. Chung,
Yaewon Kim,
Jeremy W. Gordon,
Hsin-Yu Chen,
Adam W. Autry,
Philip M. Lee,
Jasmine Y. Hu,
Chou T. Tan,
Chris Suszczynski,
Susan M. Chang,
Javier E. Villanueva-Meyer,
Robert A. Bok,
Peder E.Z. Larson,
Duan Xu,
Yan Li,
Daniel B. Vigneron
Affiliations
Brian T. Chung
Department of Radiology and Biomedical Imaging, University of California, 1700 Fourth Street, Byers Hall Suite 102, San Francisco, CA 94158, USA; UCSF – UC Berkeley Graduate Program in Bioengineering, University of California, USA
Yaewon Kim
Department of Radiology and Biomedical Imaging, University of California, 1700 Fourth Street, Byers Hall Suite 102, San Francisco, CA 94158, USA; Corresponding author.
Jeremy W. Gordon
Department of Radiology and Biomedical Imaging, University of California, 1700 Fourth Street, Byers Hall Suite 102, San Francisco, CA 94158, USA
Hsin-Yu Chen
Department of Radiology and Biomedical Imaging, University of California, 1700 Fourth Street, Byers Hall Suite 102, San Francisco, CA 94158, USA
Adam W. Autry
Department of Radiology and Biomedical Imaging, University of California, 1700 Fourth Street, Byers Hall Suite 102, San Francisco, CA 94158, USA
Philip M. Lee
Department of Radiology and Biomedical Imaging, University of California, 1700 Fourth Street, Byers Hall Suite 102, San Francisco, CA 94158, USA; UCSF – UC Berkeley Graduate Program in Bioengineering, University of California, USA
Jasmine Y. Hu
Department of Radiology and Biomedical Imaging, University of California, 1700 Fourth Street, Byers Hall Suite 102, San Francisco, CA 94158, USA; UCSF – UC Berkeley Graduate Program in Bioengineering, University of California, USA
Department of Neurological Surgery, University of California, San Francisco, CA 94158, USA
Javier E. Villanueva-Meyer
Department of Radiology and Biomedical Imaging, University of California, 1700 Fourth Street, Byers Hall Suite 102, San Francisco, CA 94158, USA
Robert A. Bok
Department of Radiology and Biomedical Imaging, University of California, 1700 Fourth Street, Byers Hall Suite 102, San Francisco, CA 94158, USA
Peder E.Z. Larson
Department of Radiology and Biomedical Imaging, University of California, 1700 Fourth Street, Byers Hall Suite 102, San Francisco, CA 94158, USA; UCSF – UC Berkeley Graduate Program in Bioengineering, University of California, USA
Duan Xu
Department of Radiology and Biomedical Imaging, University of California, 1700 Fourth Street, Byers Hall Suite 102, San Francisco, CA 94158, USA; UCSF – UC Berkeley Graduate Program in Bioengineering, University of California, USA
Yan Li
Department of Radiology and Biomedical Imaging, University of California, 1700 Fourth Street, Byers Hall Suite 102, San Francisco, CA 94158, USA
Daniel B. Vigneron
Department of Radiology and Biomedical Imaging, University of California, 1700 Fourth Street, Byers Hall Suite 102, San Francisco, CA 94158, USA; UCSF – UC Berkeley Graduate Program in Bioengineering, University of California, USA; Department of Neurological Surgery, University of California, San Francisco, CA 94158, USA
Hyperpolarized (HP) 13C Magnetic Resonance Imaging (MRI) was applied for the first time to image and quantify the uptake and metabolism of [2–13C]pyruvate in the human brain to provide new metabolic information on cerebral energy metabolism. HP [2–13C]pyruvate was injected intravenously and imaged in 5 healthy human volunteer exams with whole brain coverage in a 1-minute acquisition using a specialized spectral-spatial multi-slice echoplanar imaging (EPI) pulse sequence to acquire 13C-labeled volumetric and dynamic images of [2–13C]pyruvate and downstream metabolites [5–13C]glutamate and [2–13C]lactate. Metabolic ratios and apparent conversion rates of pyruvate-to-lactate (kPL) and pyruvate-to-glutamate (kPG) were quantified to investigate simultaneously glycolytic and oxidative metabolism in a single injection.
