Frontiers in Endocrinology (Jul 2024)
The pathogenic mechanism of monosodium urate crystal-induced kidney injury in a rat model
- Delun Li,
- Delun Li,
- Delun Li,
- Delun Li,
- Yimeng Li,
- Yimeng Li,
- Yimeng Li,
- Yimeng Li,
- Xuesheng Chen,
- Xuesheng Chen,
- Xuesheng Chen,
- Xuesheng Chen,
- Jianting Ouyang,
- Jianting Ouyang,
- Jianting Ouyang,
- Jianting Ouyang,
- Danyao Lin,
- Danyao Lin,
- Danyao Lin,
- Danyao Lin,
- Qiaoru Wu,
- Qiaoru Wu,
- Qiaoru Wu,
- Qiaoru Wu,
- Xinwen Fu,
- Xinwen Fu,
- Xinwen Fu,
- Xinwen Fu,
- Haohao Quan,
- Haohao Quan,
- Haohao Quan,
- Haohao Quan,
- Xiaowan Wang,
- Xiaowan Wang,
- Xiaowan Wang,
- Xiaowan Wang,
- Shouhai Wu,
- Shouhai Wu,
- Shouhai Wu,
- Shouhai Wu,
- Siyu Yuan,
- Siyu Yuan,
- Siyu Yuan,
- Anqi Liu,
- Jiaxiong Zhao,
- Jiaxiong Zhao,
- Xiaowu Liu,
- Xiaowu Liu,
- Gangxing Zhu,
- Gangxing Zhu,
- Chuang Li,
- Chuang Li,
- Chuang Li,
- Chuang Li,
- Wei Mao,
- Wei Mao,
- Wei Mao,
- Wei Mao
Affiliations
- Delun Li
- State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, China
- Delun Li
- Department of Nephrology, The Second Affiliated Hospital of Guangzhou University of ChineseMedicine (Guangdong Provincial Hospital of Chinese Medicine), Guangzhou, China
- Delun Li
- State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China
- Delun Li
- Nephrology Institute of Guangdong Provincial Academy of Chinese Medical Sciences (NIGH-CM), Guangzhou, China
- Yimeng Li
- State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, China
- Yimeng Li
- Department of Nephrology, The Second Affiliated Hospital of Guangzhou University of ChineseMedicine (Guangdong Provincial Hospital of Chinese Medicine), Guangzhou, China
- Yimeng Li
- State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China
- Yimeng Li
- Nephrology Institute of Guangdong Provincial Academy of Chinese Medical Sciences (NIGH-CM), Guangzhou, China
- Xuesheng Chen
- State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, China
- Xuesheng Chen
- Department of Nephrology, The Second Affiliated Hospital of Guangzhou University of ChineseMedicine (Guangdong Provincial Hospital of Chinese Medicine), Guangzhou, China
- Xuesheng Chen
- State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China
- Xuesheng Chen
- Nephrology Institute of Guangdong Provincial Academy of Chinese Medical Sciences (NIGH-CM), Guangzhou, China
- Jianting Ouyang
- State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, China
- Jianting Ouyang
- Department of Nephrology, The Second Affiliated Hospital of Guangzhou University of ChineseMedicine (Guangdong Provincial Hospital of Chinese Medicine), Guangzhou, China
- Jianting Ouyang
- State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China
- Jianting Ouyang
- Nephrology Institute of Guangdong Provincial Academy of Chinese Medical Sciences (NIGH-CM), Guangzhou, China
- Danyao Lin
- State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, China
- Danyao Lin
- Department of Nephrology, The Second Affiliated Hospital of Guangzhou University of ChineseMedicine (Guangdong Provincial Hospital of Chinese Medicine), Guangzhou, China
- Danyao Lin
- State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China
- Danyao Lin
- Nephrology Institute of Guangdong Provincial Academy of Chinese Medical Sciences (NIGH-CM), Guangzhou, China
- Qiaoru Wu
- State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, China
- Qiaoru Wu
- Department of Nephrology, The Second Affiliated Hospital of Guangzhou University of ChineseMedicine (Guangdong Provincial Hospital of Chinese Medicine), Guangzhou, China
- Qiaoru Wu
- State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China
- Qiaoru Wu
- Nephrology Institute of Guangdong Provincial Academy of Chinese Medical Sciences (NIGH-CM), Guangzhou, China
- Xinwen Fu
- State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, China
- Xinwen Fu
- Department of Nephrology, The Second Affiliated Hospital of Guangzhou University of ChineseMedicine (Guangdong Provincial Hospital of Chinese Medicine), Guangzhou, China
- Xinwen Fu
- State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China
- Xinwen Fu
- Nephrology Institute of Guangdong Provincial Academy of Chinese Medical Sciences (NIGH-CM), Guangzhou, China
- Haohao Quan
- State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, China
- Haohao Quan
- Department of Nephrology, The Second Affiliated Hospital of Guangzhou University of ChineseMedicine (Guangdong Provincial Hospital of Chinese Medicine), Guangzhou, China
- Haohao Quan
- State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China
- Haohao Quan
- Nephrology Institute of Guangdong Provincial Academy of Chinese Medical Sciences (NIGH-CM), Guangzhou, China
- Xiaowan Wang
- State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, China
- Xiaowan Wang
- Department of Nephrology, The Second Affiliated Hospital of Guangzhou University of ChineseMedicine (Guangdong Provincial Hospital of Chinese Medicine), Guangzhou, China
- Xiaowan Wang
- State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China
- Xiaowan Wang
- Nephrology Institute of Guangdong Provincial Academy of Chinese Medical Sciences (NIGH-CM), Guangzhou, China
- Shouhai Wu
- State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, China
- Shouhai Wu
- Department of Nephrology, The Second Affiliated Hospital of Guangzhou University of ChineseMedicine (Guangdong Provincial Hospital of Chinese Medicine), Guangzhou, China
- Shouhai Wu
- State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China
- Shouhai Wu
- Nephrology Institute of Guangdong Provincial Academy of Chinese Medical Sciences (NIGH-CM), Guangzhou, China
- Siyu Yuan
- Ministry of Education Key Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Tianjin University of Traditional Chinese Medicine, Tianjin, China
- Siyu Yuan
- School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin, China
- Siyu Yuan
- State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China
- Anqi Liu
- Cadre Department, Guizhou Provincial People’s Hospital, Guizhou, China
- Jiaxiong Zhao
- State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, China
- Jiaxiong Zhao
- Department of Nephrology, The Second Affiliated Hospital of Guangzhou University of ChineseMedicine (Guangdong Provincial Hospital of Chinese Medicine), Guangzhou, China
- Xiaowu Liu
- State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, China
- Xiaowu Liu
- Department of Nephrology, The Second Affiliated Hospital of Guangzhou University of ChineseMedicine (Guangdong Provincial Hospital of Chinese Medicine), Guangzhou, China
- Gangxing Zhu
- State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, China
- Gangxing Zhu
- Department of Nephrology, The Second Affiliated Hospital of Guangzhou University of ChineseMedicine (Guangdong Provincial Hospital of Chinese Medicine), Guangzhou, China
- Chuang Li
- State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, China
- Chuang Li
- Department of Nephrology, The Second Affiliated Hospital of Guangzhou University of ChineseMedicine (Guangdong Provincial Hospital of Chinese Medicine), Guangzhou, China
- Chuang Li
- State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China
- Chuang Li
- Nephrology Institute of Guangdong Provincial Academy of Chinese Medical Sciences (NIGH-CM), Guangzhou, China
- Wei Mao
- State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, China
- Wei Mao
- Department of Nephrology, The Second Affiliated Hospital of Guangzhou University of ChineseMedicine (Guangdong Provincial Hospital of Chinese Medicine), Guangzhou, China
- Wei Mao
- State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China
- Wei Mao
- Nephrology Institute of Guangdong Provincial Academy of Chinese Medical Sciences (NIGH-CM), Guangzhou, China
- DOI
- https://doi.org/10.3389/fendo.2024.1416996
- Journal volume & issue
-
Vol. 15
Abstract
Objective(MSU) crystals usually in the kidney tubules especially collecting ducts in the medulla. Previous animal models have not fully reproduced the impact of MSU on kidneys under non-hyperuricemic conditions.MethodsIn the group treated with MSU, the upper pole of the rat kidney was injected intrarenally with 50 mg/kg of MSU, while the lower pole was injected with an equivalent volume of PBS solution. The body weight and kidney mass of the rats were observed and counted. H&E staining was used to observe the pathological damage of the kidney and to count the number of inflammatory cells. Masoon staining was used to observe the interstitial fibrosis in the kidneys of the rat model. Flow cytometric analysis was used for counting inflammatory cells in rats. ElISA was used to measure the concentration of serum and urine uric acid, creatinine and urea nitrogen in rats.ResultsAt the MSU injection site, a significantly higher infiltration of inflammatory cells and a substantial increase in the area of interstitial fibrosis compared to the control group and the site of PBS injection were observed. The serum creatinine level was significantly increased in the MSU group. However, there were no significant differences in the rats’ general conditions or blood inflammatory cell counts when compared to the control group.ConclusionThe injection of urate crystals into the kidney compromised renal function, caused local pathological damage, and increased inflammatory cell infiltration and interstitial fibrosis. Intrarenal injection of MSU crystals may result in urate nephropathy. The method of intrarenal injection did not induce surgical infection or systemic inflammatory response.
Keywords