Investigation the effect of Fe3O4 nanoparticles on liver and stress oxidative parameters at the presence of magnetic field in rat

Abstract

Objective(s): This study was designed to evaluate the effect of Fe3O4 nanoparticles at presence of a constant magnetic field on rat liver and some stress oxidative parameters.Materials and Methods: Fe3O4 nanoparticles were synthesized by co-precipitation method using iron chloride (III) and iron sulphate (II). The nanoparticles properties were studied by XRD and TEM. Fourty male wistar rats were randomly divided into four groups. First group was injected with normal saline (control). Second group was injected with Fe3O4 nanoparticles (100 mg/kg). Third group was treated under a constant magnetic field and fourth group was treated with both of Fe3O4 nanoparticles injection and constant magnetic field (all injections are intra peritoneally). Liver parameters (ALT, AST, Total protein, Bilirubin) and some stress oxidative parameters such as SOD and GPX were measured for all groups, 15 and 30 days post injection.Results: The size of the synthesized nanoparticles was determined 14 nm. The crystalline structure of the nanoparticles was spinel. Serum concentration of ALT and AST were changed in some groups compared with the control group. At the presence of constant magnetic field and iron oxide injection, the amount of total protein and bilirubin significantly increased compared with that of control group. The enzyme activity of SOD and GPX haven’t changed compared to the control group.Conclusion: The results of this investigation show that this concentration of iron oxide nanoparticles (100 mg/kg) have not irreversible toxic effects at the level of liver parameters. Also, they have not any serious effects on the SOD and GPX enzyme activity even at presence of a constant magnetic field.

Keywords

• Fe3O4
• Liver parameters
• Stress oxidative