Mitotic DNA synthesis in response to replication stress requires the sequential action of DNA polymerases zeta and delta in human cells

Wei Wu; Szymon A. Barwacz; Rahul Bhowmick; Katrine Lundgaard; Marisa M. Gonçalves Dinis; Malgorzata Clausen; Masato T. Kanemaki; Ying Liu

doi:10.1038/s41467-023-35992-5

Nature Communications (Feb 2023)

Mitotic DNA synthesis in response to replication stress requires the sequential action of DNA polymerases zeta and delta in human cells

Wei Wu,
Szymon A. Barwacz,
Rahul Bhowmick,
Katrine Lundgaard,
Marisa M. Gonçalves Dinis,
Malgorzata Clausen,
Masato T. Kanemaki,
Ying Liu

Affiliations

Wei Wu: Center for Chromosome Stability, Department of Cellular and Molecular Medicine, University of Copenhagen
Szymon A. Barwacz: Center for Chromosome Stability, Department of Cellular and Molecular Medicine, University of Copenhagen
Rahul Bhowmick: Center for Chromosome Stability, Department of Cellular and Molecular Medicine, University of Copenhagen
Katrine Lundgaard: Center for Chromosome Stability, Department of Cellular and Molecular Medicine, University of Copenhagen
Marisa M. Gonçalves Dinis: Center for Chromosome Stability, Department of Cellular and Molecular Medicine, University of Copenhagen
Malgorzata Clausen: Center for Chromosome Stability, Department of Cellular and Molecular Medicine, University of Copenhagen
Masato T. Kanemaki: Department of Chromosome Science, National Institute of Genetics, Research Organization of Information and Systems (ROIS)
Ying Liu: Center for Chromosome Stability, Department of Cellular and Molecular Medicine, University of Copenhagen

DOI: https://doi.org/10.1038/s41467-023-35992-5
Journal volume & issue: Vol. 14, no. 1
pp. 1 – 17

Abstract

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DNA replication stress can generate under-replicated DNA regions which is fixed by an atypical form of DNA repair synthesis in mitosis (MiDAS). Here the authors show that translesion and replicative DNA polymerases cooperate via the POLD3 subunit to complete MiDAS in human cells.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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