Voltage Imaging of Cardiac Cells and Tissue Using the Genetically Encoded Voltage Sensor Archon1

Sanaya N. Shroff; Shoshana L. Das; Hua-an Tseng; Jad Noueihed; Fernando Fernandez; John A. White; Christopher S. Chen; Xue Han

iScience (Apr 2020)

Voltage Imaging of Cardiac Cells and Tissue Using the Genetically Encoded Voltage Sensor Archon1

Sanaya N. Shroff,
Shoshana L. Das,
Hua-an Tseng,
Jad Noueihed,
Fernando Fernandez,
John A. White,
Christopher S. Chen,
Xue Han

Affiliations

Sanaya N. Shroff: Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA
Shoshana L. Das: Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA; Harvard-MIT Program in Health Sciences and Technology, Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA
Hua-an Tseng: Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA
Jad Noueihed: Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA
Fernando Fernandez: Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA
John A. White: Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA
Christopher S. Chen: Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA; Corresponding author
Xue Han: Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA; Corresponding author

Journal volume & issue: Vol. 23, no. 4

Abstract

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Summary: Precise measurement of action potentials (APs) is needed to observe electrical activity and cellular communication within cardiac tissue. Voltage-sensitive dyes (VSDs) are traditionally used to measure cardiac APs; however, they require acute chemical addition that prevents chronic imaging. Genetically encoded voltage indicators (GEVIs) enable long-term studies of APs without the need of chemical additions, but current GEVIs used in cardiac tissue exhibit poor kinetics and/or low signal to noise (SNR). Here, we demonstrate the use of Archon1, a recently developed GEVI, in hiPSC-derived cardiomyocytes (CMs). When expressed in CMs, Archon1 demonstrated fast kinetics comparable with patch-clamp electrophysiology and high SNR significantly greater than the VSD Di-8-ANEPPS. Additionally, Archon1 enabled monitoring of APs across multiple cells simultaneously in 3D cardiac tissues. These results highlight Archon1's capability to investigate the electrical activity of CMs in a variety of applications and its potential to probe functionally complex in vitro models, as well as in vivo systems. : Biotechnology; Bioelectronics; Technical Aspects of Cell Biology; Electronic Materials Subject Areas: Biotechnology, Bioelectronics, Technical Aspects of Cell Biology, Electronic Materials

Published in iScience

ISSN: 2589-0042 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science
Website: http://www.cell.com/iscience/home

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