Proteomics Analysis Reveals the Molecular Mechanism of MoPer1 Regulating the Development and Pathogenicity of Magnaporthe oryzae

Yue Chen; Yue Chen; Xiyang Wu; Xiyang Wu; Chunyan Chen; Qiang Huang; Chenggang Li; Xin Zhang; Xinqiu Tan; Xinqiu Tan; Deyong Zhang; Deyong Zhang; Yong Liu; Yong Liu

doi:10.3389/fcimb.2022.926771

Frontiers in Cellular and Infection Microbiology (Jun 2022)

Proteomics Analysis Reveals the Molecular Mechanism of MoPer1 Regulating the Development and Pathogenicity of Magnaporthe oryzae

Yue Chen,
Yue Chen,
Xiyang Wu,
Xiyang Wu,
Chunyan Chen,
Qiang Huang,
Chenggang Li,
Xin Zhang,
Xinqiu Tan,
Xinqiu Tan,
Deyong Zhang,
Deyong Zhang,
Yong Liu,
Yong Liu

Affiliations

Yue Chen: State Key Laboratory of Hybrid Rice and Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha, China
Yue Chen: Long Ping Branch, Graduate School of Hunan University, Changsha, China
Xiyang Wu: State Key Laboratory of Hybrid Rice and Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha, China
Xiyang Wu: Long Ping Branch, Graduate School of Hunan University, Changsha, China
Chunyan Chen: College of Plant Protection, Hunan Agricultural University, Changsha, China
Qiang Huang: College of Plant Protection, Hunan Agricultural University, Changsha, China
Chenggang Li: State Key Laboratory of Hybrid Rice and Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha, China
Xin Zhang: State Key Laboratory of Hybrid Rice and Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha, China
Xinqiu Tan: State Key Laboratory of Hybrid Rice and Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha, China
Xinqiu Tan: Long Ping Branch, Graduate School of Hunan University, Changsha, China
Deyong Zhang: State Key Laboratory of Hybrid Rice and Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha, China
Deyong Zhang: Long Ping Branch, Graduate School of Hunan University, Changsha, China
Yong Liu: State Key Laboratory of Hybrid Rice and Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha, China
Yong Liu: Long Ping Branch, Graduate School of Hunan University, Changsha, China

DOI: https://doi.org/10.3389/fcimb.2022.926771
Journal volume & issue: Vol. 12

Abstract

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Glycosylphosphatidylinositol (GPI) anchoring the protein GPI modification post-transcriptionally is commonly seen. In our previous study, MoPer1, a GPI anchoring essential factor, has a critical effect on Magnaporthe oryzae growth, pathogenicity, and conidiogenesis, but its molecular mechanism is not clear. Here, we extracted the glycoproteins from the ΔMoper1 mutant and wild-type Guy11 to analyze their differential levels by quantitative proteomic analysis of TMT markers. After background subtraction, a total of 431 proteins, with significant changes in expression, were successfully identified, and these differential proteins were involved in biological regulation, as well as cellular process and metabolic process, binding, catalytic activity, and other aspects. Moreover, we found that MoPer1 regulates the expression of 14 proteins involved in growth, development, and pathogenicity of M. oryzae. The above findings shed light on MoPer1’s underlying mechanism in regulating growth, development, and pathogenicity of M. oryzae.

Published in Frontiers in Cellular and Infection Microbiology

ISSN: 2235-2988 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/Cellular-and-Infection-Microbiology

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