Zhongguo youzhi (Feb 2024)

改性活性炭构建菌载体及其吸附玉米中 黄曲霉毒素B1 的工艺研究Construction of bacterial carrier by modified activated carbon and its adsorption of AFB1 from maize

  • 丁梓雪，常程程，杨帆，王彦钦，孟宪刚 DING Zixue, CHANG Chengcheng, YANG Fan, WANG Yanqin, MENG Xiangang

DOI
https://doi.org/10.19902/j.cnki.zgyz.1003-7969.220664
Journal volume & issue
Vol. 49, no. 2
pp. 63 – 69

Abstract

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旨在为生物处理黄曲霉毒素污染的粮食饲料提供技术支持，以活性炭（SH）为原料进行改性制备磁性活性炭（CSH），选用能高效降解黄曲霉毒素B1（AFB1）的芽孢杆菌属菌株WTX1与CSH载体共培养构建磁性菌载体（FCSH），脱除受污染玉米中的AFB1，对CSH进行BET比表面积、氮吸附-脱附等温线及其对AFB1的等温吸附曲线分析，考察FCSH构建条件和FCSH吸附条件对AFB1脱除效果的影响，并利用傅里叶变换红外光谱（FTIR）和扫描电镜对吸附AFB1前后的FCSH进行表征。结果表明：CSH为大孔结构，其对AFB1的等温吸附符合Freundlich方程；在固定化时间16 h、固定化转速和吸附转速160 r/min、固定化温度和吸附温度35 ℃、吸附pH 8、振荡时间48 h条件下，FCSH对AFB1脱除率为97.45%；通过FTIR及扫描电镜证实了FCSH对AFB1的吸附作用，且FCSH对AFB1的吸附效果强于CSH。综上，所制备的FCSH能高效脱除玉米中的AFB1。Aiming to provide technical support for biological treatment of aflatoxin-contaminated grain feed, magnetic activated carbon (CSH) was prepared through modification from activated carbon(SH), and the strain of Bacillus spp. WTX1, which was capable of efficiently degrading aflatoxin B1 (AFB1), was selected to co-culture with the CSH carrier to construct the magnetic bacterial carrier (FCSH) for the removal of AFB1 from contaminated maize. BET specific surface area, nitroqen adsorption-desorption isotherms and isotherm adsorption curves for AFB1 of CSH were analyzed, and the effect of FCSH construction conditions and FCSH adsorption conditions on the effect of AFB1 removal were investigated. Fourier transform infrared spectrum (FTIR) and scanning electron microscope were used to characterize FCSH before and after adsorption of AFB1. The results showed that CSH had a macroporous structure, and its isothermal adsorption of AFB1 conformed to the Freundlich equation. The removal rate of AFB1 by FCSH could reach 97.45% under the conditions of immobilization time 16 h, immobilization and adsorption speed 160 r/min, immobilization and adsorption temperature 35 ℃, adsorption pH 8, and shaking time 48 h. The adsorption of FCSH on AFB1 was confirmed by FTIR and scanning electron microscope, and the adsorption effect of FCSH on AFB1 was stronger than that of CSH.In conclusion, the prepared FCSH can effectively remove AFB1 from maize.

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