Jornal Brasileiro de Patologia e Medicina Laboratorial (Jun 2015)

Molecular characterization of Enterobacteriaceae resistant to carbapenem antimicrobials

  • Jaciane B. Marques,
  • Pauline C. Bonez,
  • Vanessa A. Agertt,
  • Vanessa C. Flores,
  • Tanise V. Dalmolin,
  • Grazielle G. Rossi,
  • Nara Lucia F. Dal Forno,
  • Bianca V. Bianchini,
  • Caren R. Mizdal,
  • Fallon S. Siqueira,
  • Roselene A. Righi,
  • Bettina H. Meneghetti,
  • Priscila A. Trindade,
  • Roberto C. V. Santos,
  • Marli M. A. Campos

DOI
https://doi.org/10.5935/1676-2444.20150028
Journal volume & issue
Vol. 51, no. 3
pp. 162 – 165

Abstract

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ABSTRACT The present study aimed to genotypically and phenotypically characterize clinical isolates of carbapenem-resistant Enterobacteriaceae collected from inpatients at the University Hospital of Santa Maria, during seven months. Among the clinical isolates subjected to the modified Hodge test (MHT), 62.5% were positive, indicating possible production of carbapenemase. Polymerase chain reaction (PCR) demonstrated that blaKPC was the most frequently found gene (31%), followed by blaIMP (12.5%). Combined use of the methods is needed to identify carbapenem resistance in enterobacteria to prevent their spread and control the infections caused by these organisms.

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