Differences in the Pro/Antioxidative Status and Cellular Stress Response in Elderly Women after 6 Weeks of Exercise Training Supported by 1000 mg of Vitamin C Supplementation
Małgorzata Żychowska,
Ewa Sadowska-Krępa,
Elisabetta Damiani,
Luca Tiano,
Ewa Ziemann,
Alicja Nowak-Zaleska,
Patrycja Lipińska,
Anna Piotrowska,
Olga Czerwińska-Ledwig,
Wanda Pilch,
Jędrzej Antosiewicz
Affiliations
Małgorzata Żychowska
Department of Biological Foundations of Physical Culture, Kazimierz Wielki University, 85-091 Bydgoszcz, Poland
Ewa Sadowska-Krępa
Institute of Sport Sciences, Jerzy Kukuczka Academy of Physical Education, 40-065 Katowice, Poland
Elisabetta Damiani
Department of Life and Environmental Sciences, Marche Polytechnic University, 60121 Ancona, Italy
Luca Tiano
Department of Life and Environmental Sciences, Marche Polytechnic University, 60121 Ancona, Italy
Ewa Ziemann
Department of Athletics, Strength and Conditioning, Poznan University of Physical Education, 61-871 Poznan, Poland
Alicja Nowak-Zaleska
Department of Biological Foundations of Physical Culture, Kazimierz Wielki University, 85-091 Bydgoszcz, Poland
Patrycja Lipińska
Department of Biological Foundations of Physical Culture, Kazimierz Wielki University, 85-091 Bydgoszcz, Poland
Anna Piotrowska
Institute of Basics Sciences, Faculty of Physiotherapy, University of Physical Education, 31-571 Kraków, Poland
Olga Czerwińska-Ledwig
Institute of Basics Sciences, Faculty of Physiotherapy, University of Physical Education, 31-571 Kraków, Poland
Wanda Pilch
Institute of Basics Sciences, Faculty of Physiotherapy, University of Physical Education, 31-571 Kraków, Poland
Jędrzej Antosiewicz
Department of Bioenergetics and Exercise Physiology, Medical University of Gdansk, 80-210 Gdansk, Poland
Vitamin C supplementation and exercise influence pro/antioxidative status and the cellular stress response. We tested the effects of exercise training for 6 weeks, supported by 1000 mg of vitamin C supplementation in elderly women. Thirty-six women were divided into two groups: a control group (CON) (n = 18, age 69.4 ± 6.4 years, 70.4 ±10.4 kg body mass) and a supplemented group (SUPP) (n = 18, aged 67.7 ± 5.6 years, body mass 71.46 ± 5.39 kg). Blood samples were taken twice (at baseline and 24 h after the whole period of training), in order to determine vitamin C concentration, the total oxidative status/capacity (TOS/TOC), total antioxidant status/capacity (TAS/TAC), and gene expression associated with cellular stress response: encoding heat shock factor (HSF1), heat shock protein 70 (HSPA1A), heat shock protein 27 (HSPB1), and tumor necrosis factor alpha (TNF-α). We observed a significant increase in TOS/TOC, TAS/TAC, and prooxidant/antioxidant balance in the SUPP group. There was a significant decrease in HSPA1A in the CON group and a different tendency in the expression of HSF1 and TNF-α between groups. In conclusion, vitamin C supplementation enhanced the pro-oxidation in elderly women with a normal plasma vitamin C concentration and influenced minor changes in training adaptation gene expression.
