Nature Communications (Sep 2020)
Segmented flow generator for serial crystallography at the European X-ray free electron laser
- Austin Echelmeier,
- Jorvani Cruz Villarreal,
- Marc Messerschmidt,
- Daihyun Kim,
- Jesse D. Coe,
- Darren Thifault,
- Sabine Botha,
- Ana Egatz-Gomez,
- Sahir Gandhi,
- Gerrit Brehm,
- Chelsie E. Conrad,
- Debra T. Hansen,
- Caleb Madsen,
- Saša Bajt,
- J. Domingo Meza-Aguilar,
- Dominik Oberthür,
- Max O. Wiedorn,
- Holger Fleckenstein,
- Derek Mendez,
- Juraj Knoška,
- Jose M. Martin-Garcia,
- Hao Hu,
- Stella Lisova,
- Aschkan Allahgholi,
- Yaroslav Gevorkov,
- Kartik Ayyer,
- Steve Aplin,
- Helen Mary Ginn,
- Heinz Graafsma,
- Andrew J. Morgan,
- Dominic Greiffenberg,
- Alexander Klujev,
- Torsten Laurus,
- Jennifer Poehlsen,
- Ulrich Trunk,
- Davide Mezza,
- Bernd Schmidt,
- Manuela Kuhn,
- Raimund Fromme,
- Jolanta Sztuk-Dambietz,
- Natascha Raab,
- Steffen Hauf,
- Alessandro Silenzi,
- Thomas Michelat,
- Chen Xu,
- Cyril Danilevski,
- Andrea Parenti,
- Leonce Mekinda,
- Britta Weinhausen,
- Grant Mills,
- Patrik Vagovic,
- Yoonhee Kim,
- Henry Kirkwood,
- Richard Bean,
- Johan Bielecki,
- Stephan Stern,
- Klaus Giewekemeyer,
- Adam R. Round,
- Joachim Schulz,
- Katerina Dörner,
- Thomas D. Grant,
- Valerio Mariani,
- Anton Barty,
- Adrian P. Mancuso,
- Uwe Weierstall,
- John C. H. Spence,
- Henry N. Chapman,
- Nadia Zatsepin,
- Petra Fromme,
- Richard A. Kirian,
- Alexandra Ros
Affiliations
- Austin Echelmeier
- School of Molecular Sciences, Arizona State University
- Jorvani Cruz Villarreal
- School of Molecular Sciences, Arizona State University
- Marc Messerschmidt
- School of Molecular Sciences, Arizona State University
- Daihyun Kim
- School of Molecular Sciences, Arizona State University
- Jesse D. Coe
- School of Molecular Sciences, Arizona State University
- Darren Thifault
- School of Molecular Sciences, Arizona State University
- Sabine Botha
- Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University
- Ana Egatz-Gomez
- School of Molecular Sciences, Arizona State University
- Sahir Gandhi
- School of Molecular Sciences, Arizona State University
- Gerrit Brehm
- Institute for X-Ray Physics, University of Göttingen
- Chelsie E. Conrad
- School of Molecular Sciences, Arizona State University
- Debra T. Hansen
- Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University
- Caleb Madsen
- Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University
- Saša Bajt
- Deutsches Elektronen-Synchrotron
- J. Domingo Meza-Aguilar
- European XFEL
- Dominik Oberthür
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron
- Max O. Wiedorn
- Centre for Ultrafast Imaging, Universität Hamburg
- Holger Fleckenstein
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron
- Derek Mendez
- Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University
- Juraj Knoška
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron
- Jose M. Martin-Garcia
- School of Molecular Sciences, Arizona State University
- Hao Hu
- Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University
- Stella Lisova
- Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University
- Aschkan Allahgholi
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron
- Yaroslav Gevorkov
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron
- Kartik Ayyer
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron
- Steve Aplin
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron
- Helen Mary Ginn
- Division of Structural Biology, University of Oxford
- Heinz Graafsma
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron
- Andrew J. Morgan
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron
- Dominic Greiffenberg
- Paul Scherrer Institute
- Alexander Klujev
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron
- Torsten Laurus
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron
- Jennifer Poehlsen
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron
- Ulrich Trunk
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron
- Davide Mezza
- Paul Scherrer Institute
- Bernd Schmidt
- Paul Scherrer Institute
- Manuela Kuhn
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron
- Raimund Fromme
- School of Molecular Sciences, Arizona State University
- Jolanta Sztuk-Dambietz
- European XFEL
- Natascha Raab
- European XFEL
- Steffen Hauf
- European XFEL
- Alessandro Silenzi
- European XFEL
- Thomas Michelat
- European XFEL
- Chen Xu
- European XFEL
- Cyril Danilevski
- European XFEL
- Andrea Parenti
- European XFEL
- Leonce Mekinda
- European XFEL
- Britta Weinhausen
- European XFEL
- Grant Mills
- European XFEL
- Patrik Vagovic
- European XFEL
- Yoonhee Kim
- European XFEL
- Henry Kirkwood
- European XFEL
- Richard Bean
- European XFEL
- Johan Bielecki
- European XFEL
- Stephan Stern
- European XFEL
- Klaus Giewekemeyer
- European XFEL
- Adam R. Round
- European XFEL
- Joachim Schulz
- European XFEL
- Katerina Dörner
- European XFEL
- Thomas D. Grant
- Department of Structural Biology, Jacobs School of Medicine and Biomedical Sciences, SUNY University at Buffalo
- Valerio Mariani
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron
- Anton Barty
- Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron
- Adrian P. Mancuso
- European XFEL
- Uwe Weierstall
- Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University
- John C. H. Spence
- Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University
- Henry N. Chapman
- Centre for Ultrafast Imaging, Universität Hamburg
- Nadia Zatsepin
- Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University
- Petra Fromme
- School of Molecular Sciences, Arizona State University
- Richard A. Kirian
- Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University
- Alexandra Ros
- School of Molecular Sciences, Arizona State University
- DOI
- https://doi.org/10.1038/s41467-020-18156-7
- Journal volume & issue
-
Vol. 11,
no. 1
pp. 1 – 10
Abstract
Due to the pulsed nature of X-ray free electron laser (XFEL) instruments the majority of protein crystals, which are injected using continuous jet injection techniques are wasted. Here, the authors present a microfluidic device to deliver aqueous protein crystal laden droplets segmented with an immiscible oil and demonstrate that with this device an approx. 60% reduction in sample waste was achieved for data collection of 3-deoxy-D-manno-octulosonate 8-phosphate synthase crystals at the EuXFEL.
