Existence and distribution of the microbiome in tumour tissues of children with hepatoblastoma
Jinghua Cui,
Xiaoran Li,
Qun Zhang,
Bing Du,
Zanbo Ding,
Chao Yan,
Guanhua Xue,
Lin Gan,
Junxia Feng,
Zheng Fan,
Ziying Xu,
Zihui Yu,
Tongtong Fu,
Yanling Feng,
Hanqing Zhao,
Yiming Kong,
Xiaohu Cui,
Ziyan Tian,
Quanda Liu,
Jing Yuan
Affiliations
Jinghua Cui
Capital Institute of Pediatrics, Beijing, 100020, China
Xiaoran Li
Postgraduate Base of the PLA Rocket Force Medical Center, Jinzhou Medical University, Jinzhou, 121001, Liaoning Province, China; Department of Hematology and Oncology, 155th Hospital of Kaifeng, Kaifeng, 475003, Henan Province, China
Qun Zhang
Beijing Ditan Hospital, Capital Medical University, Beijing, 100015, China
Bing Du
Capital Institute of Pediatrics, Beijing, 100020, China
Zanbo Ding
Capital Institute of Pediatrics, Beijing, 100020, China
Chao Yan
Capital Institute of Pediatrics, Beijing, 100020, China
Guanhua Xue
Capital Institute of Pediatrics, Beijing, 100020, China
Lin Gan
Capital Institute of Pediatrics, Beijing, 100020, China
Junxia Feng
Capital Institute of Pediatrics, Beijing, 100020, China
Zheng Fan
Capital Institute of Pediatrics, Beijing, 100020, China
Ziying Xu
Capital Institute of Pediatrics, Beijing, 100020, China
Zihui Yu
Capital Institute of Pediatrics, Beijing, 100020, China
Tongtong Fu
Capital Institute of Pediatrics, Beijing, 100020, China
Yanling Feng
Capital Institute of Pediatrics, Beijing, 100020, China
Hanqing Zhao
Capital Institute of Pediatrics, Beijing, 100020, China
Yiming Kong
Capital Institute of Pediatrics, Beijing, 100020, China
Xiaohu Cui
Capital Institute of Pediatrics, Beijing, 100020, China
Ziyan Tian
Capital Institute of Pediatrics, Beijing, 100020, China
Quanda Liu
Postgraduate Base of the PLA Rocket Force Medical Center, Jinzhou Medical University, Jinzhou, 121001, Liaoning Province, China; Department of General Surgery, Guang'an Men Hospital, China Academy of Chinese Medical Sciences, Beijing, 100053, China; Corresponding author. Postgraduate Base of the PLA Rocket Force Medical Center, Jinzhou Medical University, Jinzhou, 121001, Liaoning Province, China.
Jing Yuan
Capital Institute of Pediatrics, Beijing, 100020, China; Corresponding author.
Cancer microbiota have recently been demonstrated in several cancer types. The microbiome enhances inflammation in the cancer microenvironment and affects the disease pathology by regulating tumourigenesis, cancer progression, and chemotherapy resistance. Hepatoblastoma (HB), the most common childhood malignant tumour, is a malignant embryonic tumour. However, the pathogenesis and molecular basis of HB remain poorly understood. In this study, to explore the existence and distribution of the microbiome in tumour tissues and adjacent non-tumour tissues of children with HB, we mainly performed 16S rDNA sequencing, and the results showed that the diversity and abundance of the microbiome in children with HB were significantly different between HB tumours and adjacent non-tumour tissues (p < 0.01). At the phylum level, the dominant microbiome in the tumour tissues were Proteobacteria, Bacteroidetes, and Firmicutes. At the genus level, Ruminococcus was more abundant in HB tumours than in the adjacent non-tumour tissues. Simultaneously, the abundances of Bacteroides, Parabacteroides, Lachnospiracea-NK4A136, and Alistipes in HB tumours were lower than those in the adjacent non-tumour tissues. In addition, Romboutsia strongly correlated with alpha-fetoprotein, an important indicator of HB. Sphingomonas was abundant in primary HB tumours, whereas Oscillibacter and Pandoraea were abundant in metastatic HB tumours. However, whether these bacteria are associated with HB needs further evaluation. Therefore, we identified the microbiome that correlated with the occurrence and development of HB. Ruminococcus and Romboutsia were identified as potential bacterial markers of HB tumours. To conclude, we found that HB also contains cancer microbiome, and it is necessary to shed light on the microbiome characteristics of HB in the future.
