Glucose- and sucrose-signaling modules regulate the Arabidopsis juvenile-to-adult phase transition
Lai-Sheng Meng,
Qin-Xin Bao,
Xin-Rong Mu,
Chen Tong,
Xiao-Ying Cao,
Jin-Jin Huang,
Li-Na Xue,
Chang-Yue Liu,
Yue Fei,
Gary J. Loake
Affiliations
Lai-Sheng Meng
The Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu 221116, People’s Republic of China; Corresponding author
Qin-Xin Bao
The Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu 221116, People’s Republic of China
Xin-Rong Mu
The Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu 221116, People’s Republic of China
Chen Tong
The Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu 221116, People’s Republic of China
Xiao-Ying Cao
The Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu 221116, People’s Republic of China
Jin-Jin Huang
The Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu 221116, People’s Republic of China
Li-Na Xue
The Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu 221116, People’s Republic of China
Chang-Yue Liu
The Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu 221116, People’s Republic of China
Yue Fei
The Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu 221116, People’s Republic of China
Gary J. Loake
Jiangsu Normal University–Edinburgh University, Centre for Transformative Biotechnology of Medicinal and Food Plants, Jiangsu Normal University, 101 Shanghai Road, Xuzhou, People’s Republic of China; Institute of Molecular Plant Sciences, School of Biological Sciences, Edinburgh University, King’s Buildings, Mayfield Road, Edinburgh EH9 3JR, UK; Corresponding author
Summary: CINV1, converting sucrose into glucose and fructose, is a key entry of carbon into cellular metabolism, and HXK1 functions as a pivotal sensor for glucose. Exogenous sugars trigger the Arabidopsis juvenile-to-adult phase transition via a miR156A/SPL module. However, the endogenous factors that regulate this process remain unclear. In this study, we show that sucrose specifically induced the PAP1 transcription factor directly and positively controls CINV1 activity. Furthermore, we identify a glucose feed-forward loop (sucrose-CINV1-glucose-HXK1-miR156-SPL9-PAP1-CINV1-glucose) that controls CINV1 activity to convert sucrose into glucose signaling to dynamically control the juvenile-to-adult phase transition. Moreover, PAP1 directly binds to the SPL9 promoter, activating SPL9 expression and triggering the sucrose-signaling-mediated juvenile-to-adult phase transition. Therefore, a glucose-signaling feed-forward loop and a sucrose-signaling pathway synergistically regulate the Arabidopsis juvenile-to-adult phase transition. Collectively, we identify a molecular link between the major photosynthate sucrose, the entry point of carbon into cellular metabolism, and the plant juvenile-to-adult phase transition.