CLONING AND SEQUENCING OF PGIP FROM ‘JIN SERIES’ ALMOND (PRUNUS DULCIS)

Abstract

Specific primers synthesized according to conservative regions of polygalacturonase inhibiting protein (PGIP) gene were used to amplify Prunus Dulcis genomic DNA by polymerase-chain reaction (PCR). Six bands (pgip1, pgip2, pgip3, pgip4, pgip5 and pgip6) of genes were obtained and cloned into PBS-T vector. According to the length of bands, 717bp, 864bp, 796bp were A1 (pgip1, pgip2, pgip3), A2 (pgip4), A4 (pgip5, pgip6), respectively. DNA sequences showed that the fragments taken together were the gene encoding PGIP. A2 and A3 contained two exons interrupted by one intron, which has GT-AG sequence. Its DNA and amino acid sequences were highly homologies to those from Prunus Persica; Prunus Salicina; Prunus Americana; Prunus Mume, respectively. A conserved lencinerial fragment exists in the derived protein sequence.

Keywords

• almond
• PGIP DNA
• Cloning
• Sequence