Genetics and Molecular Biology (Jan 2007)

Polymorphism of exon 2-3 of bovine major histocompatibility complex class I BoLa-A gene

  • Bharat Bhushan,
  • Biswa Nath Patra,
  • Pranab Jyoti Das,
  • Triveni Dutt,
  • Pushpendra Kumar,
  • Arjava Sharma,
  • Umang,
  • Satyabarta Dandapat,
  • Shiv Pal Singh Ahlawat

DOI
https://doi.org/10.1590/s1415-47572007000400009
Journal volume & issue
Vol. 30, no. 3
pp. 560 – 566

Abstract

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The exon 2-3 region of bovine major histocompatibility complex (MHC) class I BoLa-A gene was investigated for polymorphisms in three breeds of cattle originated in the Indian subcontinent namely Sahiwal, Tharparkar, Hariana, as well as crossbred (Bos taurus x Bos indicus) cattle and Jersey, the exotic breed (Bos taurus). The PCR amplified fragment of 714 bp showed distinct DdeI-, TaqI- and HinfI- RFLP patterns, thus confirming a higher degree of polymorphism in this region. To our knowledge this is the first report of HinfI restriction patterns for BoLa-A exon 2-3. The sequencing results revealed a number of nucleotide substitutions in this region, which resulted in amino acid changes. The present investigation confirmed that MHC class I BoLa-A exon 2-3 is highly polymorphic in cattle.

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