Establishment of SIAISi017-A, an induced pluripotent stem cell(iPSC)line from a healthy 25-year-old Chinese Hui

Yi Zhang; Jiajun Shou; Wentao Gu; Weihao Di; Jian Zhao; Ying Wang; Shixin Gu

Stem Cell Research (Apr 2022)

Establishment of SIAISi017-A, an induced pluripotent stem cell(iPSC)line from a healthy 25-year-old Chinese Hui

Yi Zhang,
Jiajun Shou,
Wentao Gu,
Weihao Di,
Jian Zhao,
Ying Wang,
Shixin Gu

Affiliations

Yi Zhang: Department of Neurosurgery, Huashan Hospital, Shanghai Medical College, Fudan University, China; Shanghai Clinical Medical Center of Neurosurgery, China; Neurosurgical Institute of Fudan University, China; Shanghai Key Laboratory of Brain Function and Restoration and Neural Regeneration, China
Jiajun Shou: Department of Neurosurgery, Huashan Hospital, Shanghai Medical College, Fudan University, China; Shanghai Clinical Medical Center of Neurosurgery, China; Neurosurgical Institute of Fudan University, China; Shanghai Key Laboratory of Brain Function and Restoration and Neural Regeneration, China
Wentao Gu: Department of Neurosurgery, Huashan Hospital, Shanghai Medical College, Fudan University, China; Shanghai Clinical Medical Center of Neurosurgery, China; Neurosurgical Institute of Fudan University, China; Shanghai Key Laboratory of Brain Function and Restoration and Neural Regeneration, China
Weihao Di: Lab of Regenerative Medicine and Neural Repair, Shanghai Institute for Advanced Immunochemical Studies, and School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China; Shanghai Clinical Research and Trial Center, Shanghai 201210, China
Jian Zhao: Lab of Regenerative Medicine and Neural Repair, Shanghai Institute for Advanced Immunochemical Studies, and School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China; Shanghai Clinical Research and Trial Center, Shanghai 201210, China
Ying Wang: Lab of Regenerative Medicine and Neural Repair, Shanghai Institute for Advanced Immunochemical Studies, and School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China; Shanghai Clinical Research and Trial Center, Shanghai 201210, China; Corresponding authors at: Department of Neurosurgery, Huashan Hospital, Shanghai Medical College, Fudan University, China.
Shixin Gu: Department of Neurosurgery, Huashan Hospital, Shanghai Medical College, Fudan University, China; Shanghai Clinical Medical Center of Neurosurgery, China; Neurosurgical Institute of Fudan University, China; Shanghai Key Laboratory of Brain Function and Restoration and Neural Regeneration, China; Corresponding authors at: Department of Neurosurgery, Huashan Hospital, Shanghai Medical College, Fudan University, China.

Journal volume & issue: Vol. 60
p. 102645

Abstract

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With the development of cytology, the establishment of cell models in vitro has become a powerful means to study the mechanism and treatment of diseases. Here we successfully generated the IPSC-derived modeling system of a 25-year-old healthy male. His peripheral blood mononuclear cells (PBMC) were reprogrammed using human OKSM (SOX2, OCT3/4, KLF4, and C-MYC) transcription factors using a non-integrated additional vector system. Immunocytochemistry demonstrated that IPSCS expressed all the markers of pluripotency and demonstrated their ability to differentiate spontaneously from three hypoderms in vitro. Karyotype is normal.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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