Scientific Reports (Dec 2022)

Molecular of Anaplasma marginale Theiler (Rickettsiales: Anaplasmataceae) in horseflies (Diptera: Tabanidae) in Uruguay

  • Gratchela D. Rodrigues,
  • Martín Lucas,
  • Hadassa Gabriela Ortiz,
  • Laís dos Santos Gonçalves,
  • Eduardo Blodorn,
  • William Borges Domingues,
  • Leandro Silva Nunes,
  • Anderson Saravia,
  • Pablo Parodi,
  • Franklin Riet-Correa,
  • Alejo Menchaca,
  • Vinicius Farias Campos,
  • Tiago Kütter Krolow,
  • Rodrigo Ferreira Krüger

DOI
https://doi.org/10.1038/s41598-022-27067-0
Journal volume & issue
Vol. 12, no. 1
pp. 1 – 7

Abstract

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Abstract Anaplasma marginale is transmitted biologically by infected ticks or mechanically by biting flies and contaminated fomites. In tick-free areas, such as southern Uruguay, horseflies could be the principal vectors of this pathogen for bovines, causing anaplasmosis. The objective of this work was to detect the presence of A. marginale by MSP-5 PCR and Sanger sequencing in the most prevalent species of horseflies obtained using different collection methods in Colonia, Tacuarembó and Paysandú, Uruguay. Eight horsefly species were tested (Dasybasis missionum, Poeciloderas lindneri, Tabanus campestris, T. claripennis, T. fuscofasciatus, T. platensis, T. tacuaremboensis and T. triangulum); four species were found to be positive for A. marginale, with D. missionum and P. lindneri having the most frequent infections, while only one individual each of T. fuscofasciatus and T. tacuaremboensis was positive. Both D. missionum and P. lindneri were positive for A. marginale in tick-free areas, and the implications are discussed in this report.