A genome-wide CRISPR-Cas9 knockout screen identifies FSP1 as the warfarin-resistant vitamin K reductase

Da-Yun Jin; Xuejie Chen; Yizhou Liu; Craig M. Williams; Lars C. Pedersen; Darrel W. Stafford; Jian-Ke Tie

doi:10.1038/s41467-023-36446-8

Nature Communications (Feb 2023)

A genome-wide CRISPR-Cas9 knockout screen identifies FSP1 as the warfarin-resistant vitamin K reductase

Da-Yun Jin,
Xuejie Chen,
Yizhou Liu,
Craig M. Williams,
Lars C. Pedersen,
Darrel W. Stafford,
Jian-Ke Tie

Affiliations

Da-Yun Jin: Biology Department, University of North Carolina at Chapel Hill
Xuejie Chen: Biology Department, University of North Carolina at Chapel Hill
Yizhou Liu: School of Chemistry and Molecular Biosciences, University of Queensland
Craig M. Williams: School of Chemistry and Molecular Biosciences, University of Queensland
Lars C. Pedersen: Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health
Darrel W. Stafford: Biology Department, University of North Carolina at Chapel Hill
Jian-Ke Tie: Biology Department, University of North Carolina at Chapel Hill

DOI: https://doi.org/10.1038/s41467-023-36446-8
Journal volume & issue: Vol. 14, no. 1
pp. 1 – 14

Abstract

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The authors develop a Vitamin K-dependent apoptotic reporter cell line for large-scale screening of enzymes associated with Vitamin K-dependent carboxylation and identify ferroptosis suppressor protein 1 (FSP1) as responsible for warfarin-resistant vitamin K reduction.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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