Medicina Clínica Práctica (Jun 2020)

Identification compound content of rabbit fishes (Siganus sp.) by using GCMS

  • Fendi Fendi,
  • Sutinah Made,
  • Rochmady Rochmady,
  • Wa Ode Muflihun,
  • Andi Dirpan,
  • Andi Nilawati Usman

Journal volume & issue
Vol. 3
p. 100101

Abstract

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Objective: This study aims to identify the content of compounds contained in flesh, skin, and liver in rabbit fishes (Siganus sp.). Methods: The study was conducted in several stages namely: the first stage, sampling of rabbit fishes (Siganus sp.); the second step, sample preparation on the flesh, skin, and liver; and the third stage, sample analysis using Pyrolysis Gas Chromatography-Mass Spectroscopy (Py-GCMS) with Shimadzu P2010 type. Results: Formamide, which is also known as methanamide, is an amide derived from formic acid, found in the flesh of rabbit fishes (Siganus sp.) with a concentration of 13.09%, and the skin of rabbit fishes (Siganus sp.) with a concentration of 8.34%. In addition, the compound methenamine is found in the skin of rabbit fishes (Siganus sp.) with a concentration of 13.71%. While on the skin of rabbit fishes (Siganus sp.) also the composition of methyl maltol with a concentration of 14.36%, and 1,4-diaza-2,5-dioxobicyclo[4.3.0]nonane with a concentration of 17.07%. In addition, the liver of rabbit fishes (Siganus sp.) contained ammonium carbamate compound with a concentration of 24.77%, dodecanoic acid with a concentration of 5.98%, and ketone with a concentration of 6.48%. Conclusion: The concentration of compounds in flesh, skin, and liver in rabbitfish (Siganus sp.) can be identified using Pyrolysis Gas Chromatography-Mass Spectroscopy (Py-GCMS) where there are a number of useful compounds, but further research is still needed.

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