Stem Cell Research (Oct 2020)
Development of a human iPSC line (SMBCi004-A) from a patient with Gaucher disease
- Qingyang Zhao,
- Yazhou Cui,
- Jing Wang,
- Liang Shi,
- Zijuan Qi,
- Jing Luan,
- Yuang Zhang,
- Xiaoyan Zhou,
- Jinxiang Han
Affiliations
- Qingyang Zhao
- Department of Orthopedic Surgery, The First Affiliated Hospital of Shandong First Medical University, Ji’nan 250014, Shandong, China; Biomedical Sciences College, Shandong Medicinal Biotechnology Centre, Shandong First Medical University & Shandong Academy of Medical Sciences, Ji’nan 250062, Shandong, China; Key Lab for Biotech-Drugs of National Health Commission, Ji’nan 250062, Shandong, China; Key Lab for Rare & Uncommon Diseases of Shandong Province, Ji’nan 250062, Shandong, China
- Yazhou Cui
- Department of Orthopedic Surgery, The First Affiliated Hospital of Shandong First Medical University, Ji’nan 250014, Shandong, China; Biomedical Sciences College, Shandong Medicinal Biotechnology Centre, Shandong First Medical University & Shandong Academy of Medical Sciences, Ji’nan 250062, Shandong, China; Key Lab for Biotech-Drugs of National Health Commission, Ji’nan 250062, Shandong, China; Key Lab for Rare & Uncommon Diseases of Shandong Province, Ji’nan 250062, Shandong, China
- Jing Wang
- Department of Orthopedic Surgery, The First Affiliated Hospital of Shandong First Medical University, Ji’nan 250014, Shandong, China; Biomedical Sciences College, Shandong Medicinal Biotechnology Centre, Shandong First Medical University & Shandong Academy of Medical Sciences, Ji’nan 250062, Shandong, China; Key Lab for Biotech-Drugs of National Health Commission, Ji’nan 250062, Shandong, China; Key Lab for Rare & Uncommon Diseases of Shandong Province, Ji’nan 250062, Shandong, China
- Liang Shi
- Department of Orthopedic Surgery, The First Affiliated Hospital of Shandong First Medical University, Ji’nan 250014, Shandong, China; Biomedical Sciences College, Shandong Medicinal Biotechnology Centre, Shandong First Medical University & Shandong Academy of Medical Sciences, Ji’nan 250062, Shandong, China; Key Lab for Biotech-Drugs of National Health Commission, Ji’nan 250062, Shandong, China; Key Lab for Rare & Uncommon Diseases of Shandong Province, Ji’nan 250062, Shandong, China
- Zijuan Qi
- Department of Orthopedic Surgery, The First Affiliated Hospital of Shandong First Medical University, Ji’nan 250014, Shandong, China; Biomedical Sciences College, Shandong Medicinal Biotechnology Centre, Shandong First Medical University & Shandong Academy of Medical Sciences, Ji’nan 250062, Shandong, China; Key Lab for Biotech-Drugs of National Health Commission, Ji’nan 250062, Shandong, China; Key Lab for Rare & Uncommon Diseases of Shandong Province, Ji’nan 250062, Shandong, China
- Jing Luan
- Department of Orthopedic Surgery, The First Affiliated Hospital of Shandong First Medical University, Ji’nan 250014, Shandong, China; Biomedical Sciences College, Shandong Medicinal Biotechnology Centre, Shandong First Medical University & Shandong Academy of Medical Sciences, Ji’nan 250062, Shandong, China; Key Lab for Biotech-Drugs of National Health Commission, Ji’nan 250062, Shandong, China; Key Lab for Rare & Uncommon Diseases of Shandong Province, Ji’nan 250062, Shandong, China
- Yuang Zhang
- Department of Orthopedic Surgery, The First Affiliated Hospital of Shandong First Medical University, Ji’nan 250014, Shandong, China; Biomedical Sciences College, Shandong Medicinal Biotechnology Centre, Shandong First Medical University & Shandong Academy of Medical Sciences, Ji’nan 250062, Shandong, China; Key Lab for Biotech-Drugs of National Health Commission, Ji’nan 250062, Shandong, China; Key Lab for Rare & Uncommon Diseases of Shandong Province, Ji’nan 250062, Shandong, China
- Xiaoyan Zhou
- Department of Orthopedic Surgery, The First Affiliated Hospital of Shandong First Medical University, Ji’nan 250014, Shandong, China; Biomedical Sciences College, Shandong Medicinal Biotechnology Centre, Shandong First Medical University & Shandong Academy of Medical Sciences, Ji’nan 250062, Shandong, China; Key Lab for Biotech-Drugs of National Health Commission, Ji’nan 250062, Shandong, China; Key Lab for Rare & Uncommon Diseases of Shandong Province, Ji’nan 250062, Shandong, China
- Jinxiang Han
- Department of Orthopedic Surgery, The First Affiliated Hospital of Shandong First Medical University, Ji’nan 250014, Shandong, China; Biomedical Sciences College, Shandong Medicinal Biotechnology Centre, Shandong First Medical University & Shandong Academy of Medical Sciences, Ji’nan 250062, Shandong, China; Key Lab for Biotech-Drugs of National Health Commission, Ji’nan 250062, Shandong, China; Key Lab for Rare & Uncommon Diseases of Shandong Province, Ji’nan 250062, Shandong, China; Corresponding author at: Department of Orthopedic Surgery, The First Affiliated Hospital of Shandong First Medical University, Ji'nan, Shandong, China.
- Journal volume & issue
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Vol. 48
p. 101989
Abstract
A human induced pluripotent stem cell (iPSC) line was generated from the urine cells of a 8-year-old female with Gaucher disease with the homozygous changes c.1448 T>C in the GBA gene. Reprogramming factors OCT4, SOX2, KLF4, and miR-302–367 were delivered using a non-integrative plasmid. Our iPSCs showed complete pluripotency, normal genetic stability, and the ability to differentiate into three germ layers.
