Optimized CRISPR tools and site-directed transgenesis towards gene drive development in Culex quinquefasciatus mosquitoes

Xuechun Feng; Víctor López Del Amo; Enzo Mameli; Megan Lee; Alena L. Bishop; Norbert Perrimon; Valentino M. Gantz

doi:10.1038/s41467-021-23239-0

Nature Communications (May 2021)

Optimized CRISPR tools and site-directed transgenesis towards gene drive development in Culex quinquefasciatus mosquitoes

Xuechun Feng,
Víctor López Del Amo,
Enzo Mameli,
Megan Lee,
Alena L. Bishop,
Norbert Perrimon,
Valentino M. Gantz

Affiliations

Xuechun Feng: Section of Cell and Developmental Biology, University of California San Diego
Víctor López Del Amo: Section of Cell and Developmental Biology, University of California San Diego
Enzo Mameli: Department of Genetics, Blavatnik Institute, Harvard Medical School
Megan Lee: Section of Cell and Developmental Biology, University of California San Diego
Alena L. Bishop: Section of Cell and Developmental Biology, University of California San Diego
Norbert Perrimon: Department of Genetics, Blavatnik Institute, Harvard Medical School
Valentino M. Gantz: Section of Cell and Developmental Biology, University of California San Diego

DOI: https://doi.org/10.1038/s41467-021-23239-0
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 13

Abstract

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Culex mosquitoes are a global vector for insect-borne diseases, though progress with genetic tools lags behind other mosquito species. Here the authors present a Cas9-based toolkit and methods that could support future gene drive development in these mosquitoes.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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