Callus formation and shoot induction from intercalar tissue of <em>Guadua angustifolia </em>Kunth adult plants branches

Abstract

Bamboo plants are largely used in building programs, furniture construction among others. Guadua angustifolia Kunth is a bamboo original from Ecuador and Colombia with certain interesting characteristics for reforestation programs. Vegetative propagation of these species is limited taking into account the desire to introduce them for production. Obtaining callus formation from the intercalar tissues of Guadua braches was the objective of this project. Starting material was disinfected using mercury bichlorine (0.2%) for a period of 10 minutes. Intercalar tissue segments were established in culture medium supplemented with picloram. Callus formation was obtained from these explants and the presence of green shoots was achieved with 6-BAP (benzylaminopurine). Key words: bamboo, temporal immersion, picloram