Stem Cell Research (Jan 2021)

Generation of an iPSC line (IMAGINi011-A) from a patient carrying a STING mutation

  • Laura Barnabei,
  • Mathieu Castela,
  • Celine Banal,
  • Nathalie Lefort,
  • Frédéric Rieux-Laucat

Journal volume & issue
Vol. 50
p. 102107

Abstract

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Mutation in STING1 gene, which encodes stimulator of type I IFN gene (STING) leads to its constitutive activation and thereby to a severe vasculopathy and sometimes a lupus-like disease. We generated induced pluripotent stem cells (iPSCs) from a patient carrying a rare heterozygous variant c.463G > A (resulting in a p.V155M substitution) in STING1. Cells from this patient, which were reprogrammed by non-integrative viral transduction, had normal karyotype, expressed pluripotency markers and were able to differentiate into the three germ cell layers.