Lethal concentration (LC50) determination of fresh water angel fish (Pterophyllum scalare) exposed to zinc oxide nanoparticles (ZnO NPs)

Abstract

The aim of this study was to determine the lethal concentration of zinc oxide nanoparticles in freshwater angelfish. Accordingly, 126 specimens of angelfish were prepared with a mean standard length of 3 ±0.51 mm and an average weight of 3.5 ± 0.3 g and after adaptation to new conditions, a toxicity test was performed in a completely randomized design with three replications for 96 hours. In addition to the control group, 6 treatments were prepared with concentrations of 0, 40, 80, 120, 160, and 200 mg/l zinc oxide nanoparticles. After the end of the experiment, the data were analyzed by probit analysis with a 95% confidence level and lethal concentrations ranging from 5 to 95 percent values ​​were calculated from the probit table. Then, the lethal concentration of 50 percent at 24, 48, 72 and 96 hours was 188.04, 119.93, 83.75, and 74.99 mg/L, respectively, by probit analysis. The results of this study show that zinc oxide nanoparticles are slightly toxic in terms of toxicity to freshwater angelfish.

Keywords

• angel fish
• probit
• toxicity
• zinc oxide
• nanoparticles