A new amperometric sucrose biosensor based on fructose dehydrogenase

Abstract

A new, ferricyanide mediated amperometric biosensor for estimation of sucrose in biological fluids is proposed based on fructose dehydrogenase coimmobilised with baker's yeast as a source of invertase on the surface of glassy carbon electrode. Potential +420 mV was applied to the electrode against saturated calomel electrode. Maximum current was observed at pH 6, linearity of response ranged from 0.01mM to 0.29mM. Concentration of potassium ferricyanide 2mM was found as the best value.

Keywords

• biosensor
• sucrose
• fructose dehydrogenase
• baker's yeas