Jichu yixue yu linchuang (Jul 2021)
LINC00607 affects the proliferation and apoptosis of A549/DDP cells through inhibiting miR-372
Abstract
Objective To explore the effect of LINC00607 on proliferation and apoptosis of lung cancer cisplatin-resistant cell line(A549/DDP) and its possible molecular mechanism. Methods The expressions of LINC00607 and miR-372 in lung cancer tissues, adjacent tissues, A549, A549/DDP cells were detected by real-time fluorescence quantitative PCR(RT-qOCR). A549/DDP cells were divided into pcDNA3.1 group, pcDNA3.1-LINC00607 group, (pcDNA3.1-LINC00607 + miR-NC)group, (pcDNA3.1-LINC00607 + miR-372)group. Methyl thiazolyl tetrazolium (MTT) assacy and colony formation experiments were used to test cell proliferation; flow cytometry was used to measure apoptosis; and Western blot was used to detect the expression levels of cleared-caspase-3 and Ki67; Dual luciferase reporter gene and RT-qPCR experiments confirmed the targeted regulation effect of LINC00607 on miR-372. Results Compared with adjacent tissues, the expression of LINC00607 in lung cancer tissues was significantly reduced, while the expression of miR-372 was significantly increased (P＜0.05). Compared with A549 cells, the expression of LINC00607 in A549/DDP cells was significantly reduced, while the expression of miR-372 was significantly increased (P＜0.05). Compared with pcDNA3.1 group, A549/DDP cell survival rate, clone formation numbers, Ki67 protein expression in pcDNA3.1-LINC00607 group were significantly decreased, and apoptosis rate and cleared-caspase-3 protein expression were significantly increased (P＜0.05). Compared with the (pcDNA3.1-LINC00607 + miR-NC)group, the A549/DDP cell survival rate, clone formation, Ki67 protein expression in the (pcDNA3.1-LINC00607 + miR-372)group were significantly increased, and the apoptosis rate, cleared-caspase-3 protein expression were significantly reduced (P＜0.05). LINC00607 targeted at and negatively regulated miR-372 expression. Conclusions LINC00607 inhibits proliferation and induces apoptosis of A549/DDP cells by down-regulating miR-372.
