Protocol for recording neural activity evoked by electrical stimulation in mice using two-photon calcium imaging

Abstract

Summary: Electrical stimulation provides a clinically viable approach for treating neurological disorders. Here, we present a protocol for recording neural activity evoked by electrical stimulation in mice using two-photon calcium imaging. We detail steps for chronically implanting a head fixation bar, a stimulating electrode, and a glass imaging window. We additionally describe the procedures for viral injections and awake head-fixed recordings.For complete details on the use and execution of this protocol, please refer to Dadarlat et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Neuroscience
• Biotechnology and bioengineering