Genome-wide linkage mapping of QTL for root hair length in a Chinese common wheat population
Feng Huang,
Zhaoyan Chen,
Dejie Du,
Panfeng Guan,
Lingling Chai,
Weilong Guo,
Zhaorong Hu,
Mingming Xin,
Huiru Peng,
Yingyin Yao,
Zhongfu Ni
Affiliations
Feng Huang
Key Laboratory of Crop Heterosis and Utilization (MOE)/State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China; National Plant Gene Research Center, Beijing 100193, China; Zhoukou Academy of Agriculture Sciences, Zhoukou 466001, Henan, China
Zhaoyan Chen
Key Laboratory of Crop Heterosis and Utilization (MOE)/State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China; National Plant Gene Research Center, Beijing 100193, China
Dejie Du
Key Laboratory of Crop Heterosis and Utilization (MOE)/State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China; National Plant Gene Research Center, Beijing 100193, China
Panfeng Guan
Key Laboratory of Crop Heterosis and Utilization (MOE)/State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China; National Plant Gene Research Center, Beijing 100193, China
Lingling Chai
Key Laboratory of Crop Heterosis and Utilization (MOE)/State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China; National Plant Gene Research Center, Beijing 100193, China
Weilong Guo
Key Laboratory of Crop Heterosis and Utilization (MOE)/State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China; National Plant Gene Research Center, Beijing 100193, China
Zhaorong Hu
Key Laboratory of Crop Heterosis and Utilization (MOE)/State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China; National Plant Gene Research Center, Beijing 100193, China
Mingming Xin
Key Laboratory of Crop Heterosis and Utilization (MOE)/State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China; National Plant Gene Research Center, Beijing 100193, China
Huiru Peng
Key Laboratory of Crop Heterosis and Utilization (MOE)/State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China; National Plant Gene Research Center, Beijing 100193, China
Yingyin Yao
Key Laboratory of Crop Heterosis and Utilization (MOE)/State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China; National Plant Gene Research Center, Beijing 100193, China
Zhongfu Ni
Key Laboratory of Crop Heterosis and Utilization (MOE)/State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China; National Plant Gene Research Center, Beijing 100193, China; Corresponding author at: Key Laboratory of Crop Heterosis and Utilization (MOE)/State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China.
Root hairs are fast growing, ephemeral tubular extensions of the root epidermis that aid nutrient and water uptake. The aim of the present study was to identify QTL for root hair length (RHL) using 227 F8 recombinant inbred lines (RILs) derived from a cross of Zhou 8425B (Z8425B) and Chinese Spring (CS), and to develop convenient molecular markers for marker-assisted breeding in wheat. Analysis of variance of root hair length showed significant differences (P 2.5) for RHL were identified on chromosomes 1B (2), 2D and 6D and four putative QTL (2 ≤ LOD ≤ 2.5) were detected on chromosomes 1A, 3A, 6B, and 7B, explaining 3.32%–6.52% of the phenotypic variance. The positive alleles for increased RHL of QTL on chromosomes 2D, 6B and 6D (QRhl.cau-2D, qRhl.cau-6B, and QRhl.cau-6D) were contributed by Z8425B, and CS contributed positive QTL alleles on chromosomes 1A (qRhl.cau-1A), 1B (QRhl.cau-1B.1 and QRhl.cau-1B.2), 3A (qRhl.cau-3A) and 7B (qRhl.cau-7B). STARP markers were developed for QRhl.cau-1B.1, QRhl.cau-2D, QRhl.cau-6D, and qRhl.cau-7B. Haplotype and association analysis indicated that the positive allele of QRhl.cau-6D had been strongly selected in Chinese wheat breeding programs. Collectively, the identified QTL for root hair length are likely to be useful for marker-assisted selection.
