Single-cell RNA sequencing reveals molecular and functional platelet bias of aged haematopoietic stem cells

Amit Grover; Alejandra Sanjuan-Pla; Supat Thongjuea; Joana Carrelha; Alice Giustacchini; Adriana Gambardella; Iain Macaulay; Elena Mancini; Tiago C. Luis; Adam Mead; Sten Eirik W. Jacobsen; Claus Nerlov

doi:10.1038/ncomms11075

Nature Communications (Mar 2016)

Single-cell RNA sequencing reveals molecular and functional platelet bias of aged haematopoietic stem cells

Amit Grover,
Alejandra Sanjuan-Pla,
Supat Thongjuea,
Joana Carrelha,
Alice Giustacchini,
Adriana Gambardella,
Iain Macaulay,
Elena Mancini,
Tiago C. Luis,
Adam Mead,
Sten Eirik W. Jacobsen,
Claus Nerlov

Affiliations

Amit Grover: MRC Molecular Hematology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford
Alejandra Sanjuan-Pla: MRC Molecular Hematology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford
Supat Thongjuea: MRC Molecular Hematology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford
Joana Carrelha: MRC Molecular Hematology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford
Alice Giustacchini: MRC Molecular Hematology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford
Adriana Gambardella: MRC Molecular Hematology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford
Iain Macaulay: MRC Molecular Hematology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford
Elena Mancini: EMBL Mouse Biology Program
Tiago C. Luis: MRC Molecular Hematology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford
Adam Mead: MRC Molecular Hematology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford
Sten Eirik W. Jacobsen: MRC Molecular Hematology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford
Claus Nerlov: MRC Molecular Hematology Unit, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford

DOI: https://doi.org/10.1038/ncomms11075
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 12

Abstract

Read online

With age, haematopoietic stem cells (HSCs) produce more myeloid than lymphoid cells, affecting adaptive immunity. By combining HSC single cell transcriptomics with functional studies, Grover et al. find that platelet production is also increased in old murine HSCs and show that the FOG-1 transcription factor contributes to the age-dependent platelet bias.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal