GMS Hygiene and Infection Control (Sep 2020)

Streptococcus agalactiae clinical isolates in Northwest Iran: antibiotic susceptibility, molecular typing, and biofilm formation

  • Shadbad, Mohammad Alipour,
  • Kafil, Hossein Samadi,
  • Rezaee, Mohammad Ahangarzadeh,
  • Farzami, Marjan Rahnamaye,
  • Dehkharghani, Alireza Dolatyar,
  • Sadeghi, Javid,
  • Gholizadeh, Pourya,
  • Khodaei, Farzaneh,
  • Aghazadeh, Mohammad

DOI
https://doi.org/10.3205/dgkh000358
Journal volume & issue
Vol. 15
p. Doc23

Abstract

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Background: Group B Streptococcus () is one of the colonizing bacteria in pregnant women which can be a causative agent of meningitis and neonatal sepsis. This organism has also been increasingly related to invasive infections in non-pregnant adults. Objective: In present study, we aimed to characterize the clonality of biofilm-producing isolates from various sources from two different clinical laboratories in Tehran, Iran.Materials and Methods: isolates were collected from community-acquired (CA) and hospital-acquired (HA) infections in pregnant and non-pregnant adults. The antimicrobial susceptibility patterns and biofilm formation ability were determined. In addition, pulse field gel electrophoresis (PFGE) was used to verify the clonal diversity of isolates.Results: Out of the 87 isolates, 15 (16.6%) formed biofilm. The antibiotic resistance rate was 98.85% for clindamycin, 98.85% for tetracycline, followed by 29.88% for erythromycin, 9.19% for moxifloxacin and 6.89% for levofloxacin. The PFGE patterns revealed a total of 16 different clusters consisting of 6 single types (STs). Conclusion: This study evaluated the biofilm formation of clinical , which may be a step towards understanding its role in pathological processes. Biofilm formation was significant only in the hypervirulent ST-17 clone. Intraclonal spread of isolates indicates that a local lineage of isolates is responsible for infection by these bacteria.

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