Expressed Sequence Tags Analysis and Design of Simple Sequence Repeats Markers from a Full-Length cDNA Library in Perilla frutescens (L.)

Eun Soo Seong; Ji Hye Yoo; Jae Hoo Choi; Chang Heum Kim; Mi Ran Jeon; Byeong Ju Kang; Jae Geun Lee; Seon Kang Choi; Bimal Kumar Ghimire; Chang Yeon Yu

doi:10.1155/2015/679548

International Journal of Genomics (Jan 2015)

Expressed Sequence Tags Analysis and Design of Simple Sequence Repeats Markers from a Full-Length cDNA Library in Perilla frutescens (L.)

Eun Soo Seong,
Ji Hye Yoo,
Jae Hoo Choi,
Chang Heum Kim,
Mi Ran Jeon,
Byeong Ju Kang,
Jae Geun Lee,
Seon Kang Choi,
Bimal Kumar Ghimire,
Chang Yeon Yu

Affiliations

Eun Soo Seong: Bioherb Research Institute, Kangwon National University, Chuncheon 200-701, Republic of Korea
Ji Hye Yoo: Department of Bioconvergence Science and Technology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea
Jae Hoo Choi: Department of Bioconvergence Science and Technology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea
Chang Heum Kim: Department of Bioconvergence Science and Technology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea
Mi Ran Jeon: Department of Bioconvergence Science and Technology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea
Byeong Ju Kang: Department of Bioconvergence Science and Technology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea
Jae Geun Lee: Hwajin Cosmetics, Hongcheon 250-807, Republic of Korea
Seon Kang Choi: Department of Agricultural Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea
Bimal Kumar Ghimire: Department of Applied Bioscience, Konkuk University, Seoul 143-701, Republic of Korea
Chang Yeon Yu: Department of Bioconvergence Science and Technology, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea

DOI: https://doi.org/10.1155/2015/679548
Journal volume & issue: Vol. 2015

Abstract

Read online

Perilla frutescens is valuable as a medicinal plant as well as a natural medicine and functional food. However, comparative genomics analyses of P. frutescens are limited due to a lack of gene annotations and characterization. A full-length cDNA library from P. frutescens leaves was constructed to identify functional gene clusters and probable EST-SSR markers via analysis of 1,056 expressed sequence tags. Unigene assembly was performed using basic local alignment search tool (BLAST) homology searches and annotated Gene Ontology (GO). A total of 18 simple sequence repeats (SSRs) were designed as primer pairs. This study is the first to report comparative genomics and EST-SSR markers from P. frutescens will help gene discovery and provide an important source for functional genomics and molecular genetic research in this interesting medicinal plant.

Published in International Journal of Genomics

ISSN: 2314-436X (Print); 2314-4378 (Online)
Publisher: Hindawi Limited
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General): Genetics
Website: https://onlinelibrary.wiley.com/journal/4140

About the journal