Zinc finger protein ZNF384 is an adaptor of Ku to DNA during classical non-homologous end-joining

Jenny Kaur Singh; Rebecca Smith; Magdalena B. Rother; Anton J. L. de Groot; Wouter W. Wiegant; Kees Vreeken; Ostiane D’Augustin; Robbert Q. Kim; Haibin Qian; Przemek M. Krawczyk; Román González-Prieto; Alfred C. O. Vertegaal; Meindert Lamers; Sébastien Huet; Haico van Attikum

doi:10.1038/s41467-021-26691-0

Nature Communications (Nov 2021)

Zinc finger protein ZNF384 is an adaptor of Ku to DNA during classical non-homologous end-joining

Jenny Kaur Singh,
Rebecca Smith,
Magdalena B. Rother,
Anton J. L. de Groot,
Wouter W. Wiegant,
Kees Vreeken,
Ostiane D’Augustin,
Robbert Q. Kim,
Haibin Qian,
Przemek M. Krawczyk,
Román González-Prieto,
Alfred C. O. Vertegaal,
Meindert Lamers,
Sébastien Huet,
Haico van Attikum

Affiliations

Jenny Kaur Singh: Department of Human Genetics, Leiden University Medical Center
Rebecca Smith: Univ Rennes, CNRS, IGDR (Institut de génétique et développement de Rennes)—UMR 6290
Magdalena B. Rother: Department of Human Genetics, Leiden University Medical Center
Anton J. L. de Groot: Department of Human Genetics, Leiden University Medical Center
Wouter W. Wiegant: Department of Human Genetics, Leiden University Medical Center
Kees Vreeken: Department of Human Genetics, Leiden University Medical Center
Ostiane D’Augustin: Univ Rennes, CNRS, IGDR (Institut de génétique et développement de Rennes)—UMR 6290
Robbert Q. Kim: Department of Cell and Chemical Biology, Leiden University Medical Center
Haibin Qian: Department of Medical Biology, Amsterdam University Medical Centers (location AMC), Cancer Center Amsterdam
Przemek M. Krawczyk: Department of Medical Biology, Amsterdam University Medical Centers (location AMC), Cancer Center Amsterdam
Román González-Prieto: Department of Medical Biology, Amsterdam University Medical Centers (location AMC), Cancer Center Amsterdam
Alfred C. O. Vertegaal: Department of Medical Biology, Amsterdam University Medical Centers (location AMC), Cancer Center Amsterdam
Meindert Lamers: Department of Medical Biology, Amsterdam University Medical Centers (location AMC), Cancer Center Amsterdam
Sébastien Huet: Univ Rennes, CNRS, IGDR (Institut de génétique et développement de Rennes)—UMR 6290
Haico van Attikum: Department of Human Genetics, Leiden University Medical Center

DOI: https://doi.org/10.1038/s41467-021-26691-0
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 21

Abstract

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Classical non-homologous end-joining (cNHEJ) is the dominant pathway used by human cells to repair DNA double-strand breaks (DSBs) and maintain genome stability. Here the authors show that PARP1-driven chromatin expansion allows the recruitment of ZNF384, which in turn recruits Ku70/Ku80 to facilitate cNHEJ.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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