Underwater instant adhesion mechanism of self-assembled amphiphilic hemostatic granular hydrogel from Andrias davidianus skin secretion
Yuqing Liu,
Yinghao Li,
Haitao Shang,
Wen Zhong,
Quan Wang,
Kibret Mequanint,
Chuhong Zhu,
Malcolm Xing,
Hong Wei
Affiliations
Yuqing Liu
Department of Mechanical Engineering, University of Manitoba, Winnipeg, MB R3T 2N2, Canada
Yinghao Li
Department of Anatomy, Key Lab for Biomechanics and Tissue Engineering of Chongqing, Army Medical University (Third Military Medical University), Chongqing, 400038, China; Chongqing Institute of Zhong Zhi Yi Gu, Shapingba District, Chongqing 400030, China
Haitao Shang
Institute of Precision Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510080, China
Wen Zhong
Department of Biosystems Engineering, University of Manitoba, Winnipeg, MB R3T 2N2, Canada
Quan Wang
Department of Civil Engineering, Shantou University, Shantou 515063, China
Kibret Mequanint
Department of Chemical and Biochemical Engineering, University of Western Ontario, London, ON N6A 5B9, Canada; School of Biomedical Engineering, University of Western Ontario, London, ON N6A 5B9, Canada
Chuhong Zhu
Department of Anatomy, Key Lab for Biomechanics and Tissue Engineering of Chongqing, Army Medical University (Third Military Medical University), Chongqing, 400038, China; State Key Laboratory of Trauma, Burn and Combined Injury, Key Lab for Biomechanics and Tissue Engineering of Chongqing, Army Medical University (Third Military Medical University), Chongqing 400038, China; Corresponding author
Malcolm Xing
Department of Mechanical Engineering, University of Manitoba, Winnipeg, MB R3T 2N2, Canada; Corresponding author
Hong Wei
Institute of Precision Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510080, China; Corresponding author
Summary: The widespread use of biological tissue adhesives for tissue repair is limited by their weak adhesion in a wet environment. Herein, we report the wet adhesion mechanism of a dry granular natural bioadhesive from Andrias davidianus skin secretion (ADS). Once contacting water, ADS granules self-assemble to form a hydrophobic hydrogel strongly bonding to wet substrates in seconds. ADS showed higher shear adhesion than current commercial tissue adhesives and an impressive 72-h underwater adhesion strength of ∼47kPa on porcine skin tissue. The assembled hydrogel in water maintained a dissipation energy of ∼8 kJ/m3, comparable to the work density of muscle, exhibiting its robustness. Unlike catechol adhesion mechanism, ADS wet adhesion mechanism is attributed to water absorption by granules, and the unique equilibrium of protein hydrophobicity, hydrogen bonding, and ionic complexation. The in vivo adhesion study demonstrated its excellent wet adhesion and hemostasis performance in a rat hepatic and cardiac hemorrhage model.
