Programmable RNA targeting with CRISPR-Cas13

Abstract

ABSTRACTThe RNA-targeting CRISPR-Cas13 system has enabled precise engineering of endogenous RNAs, significantly advancing our understanding of RNA regulation and the development of RNA-based diagnostic and therapeutic applications. This review aims to provide a summary of Cas13-based RNA targeting tools and applications, discuss limitations and challenges of existing tools and suggest potential directions for further development of the RNA targeting system.

Keywords

• CRISPR-Cas13
• RNA
• collateral activity