OncoTargets and Therapy (Mar 2020)
MiR-483 Targeted SOX3 to Suppress Glioma Cell Migration, Invasion and Promote Cell Apoptosis
Abstract
Shujing Lu,1 Zhengyang Yu,2 Xia Zhang,2 Lingling Sui2 1Department of Critical Care Medicine, Liaocheng Third People’s Hospital, Liaocheng 252000, Shandong, People’s Republic of China; 2Department of Internal Neurology, Liaocheng Third People’s Hospital, Liaocheng 252000, Shandong, People’s Republic of ChinaCorrespondence: Lingling SuiDepartment of Internal Neurology, Liaocheng Third People’s Hospital, No. 62 Weiyu Road, Liaocheng 252000, Shandong, People’s Republic of ChinaTel +86 635-8385120Email [email protected]: Glioma is the most common malignant brain tumor that has high aggressiveness. The aim of this study was to investigate the potential therapeutic targets for gliomas.Materials and Methods: Real-time quantitative polymerase chain reaction (RT-qPCR) was employed to calculate the expression of miRNA and genes. The connection between the expression of miR-483 and patients’ overall survival rate was evaluated using Kaplan–Meier analysis. In addition, the underlying mechanism was detected using luciferase assay.Results: The expression level of miR-483 was significantly decreased in glioma tissue samples and cell lines, compared to the adjacent tissues and normal cell lines. Downregulation of miR-483 or upregulation of SOX3 was associated with overall survival of glioma patients. Additionally, overexpression of miR-483 promotes cell invasion and migration and inhibits apoptosis. In addition, miR-483 directly targeted to SOX3, and the expression of miR-483 has a negative correlation with SOX3 in glioma tissues. SOX3 reversed partial functions of miR-483 on cell migration, invasion, and promoted cell apoptosis in glioma.Conclusion: MiR-483 inhibited glioma cell migration, invasion, and promoted glioma cell apoptosis by targeting SOX3. MiR-483 maybe acted as a potential target for the treatment of glioma.Keywords: miR-483, SOX3, glioma, apoptosis, invasion
