OncoTargets and Therapy (Mar 2020)
KIF4A Promotes Clear Cell Renal Cell Carcinoma (ccRCC) Proliferation in vitro and in vivo
Abstract
Guang-Hua Yang,1,* Zhi-Xing Ren,2,* Xiong Yang,3 Yan-Gang Zhang1 1Department of Urology, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Taiyuan City, Shanxi Province 030032, People’s Republic of China; 2Education and Research Center, Taiyuan Radio and Television University, Taiyuan City, Shanxi Province 030024, People’s Republic of China; 3Department of Urolith Center, Tianjin Institute of Urology, The Second Hospital of Tianjin Medical University, Tianjin 300211, People’s Republic of China*These authors contributed equally to this workCorrespondence: Guang-Hua Yang; Yan-Gang ZhangDepartment of Urology, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, No. 99 Longcheng Street, Taiyuan City, Shanxi Province 030032, People’s Republic of ChinaTel +86 351-8368114Email [email protected]; [email protected]: To evaluate the expression in human clear cell renal cell carcinoma (ccRCC) tissues and explore the effects of kinesin family member 4A (KIF4A) on ccRCC progression.Methods: GEPIA was used to evaluate the mRNA levels of KIF4A in human ccRCC tissues from TCGA database, and Immunohistochemistry (IHC) assays were performed to assess its expression in human ccRCC tissues collected in our hospital. The clinical-pathological analysis was performed to explore the correlation with KIF4A expression. The effects of KIF4A on ccRCC cell proliferation were detected through colony formation and MTT assays. Finally, the effects of KIF4A on tumor growth were measured using a mice model.Results: Bioinformation results showed the expression of KIF4A mRNA was upregulated in ccRCC tissues and high expression of KIF4A was related with poor prognosis in ccRCC patients. We also found a high expression of KIF4A in human ccRCC tissues collected in our hospital. We also found its expression level was correlated with clinical characteristics, including T stage (P=0.035*) and lymphatic metastasis (P=0.028*). We further confirmed that knockdown of KIF4A suppressed cell proliferation in HTB-47 and CRL-1932 cells. Furthermore, KIF4A contributes to tumor growth of ccRCC cells in mice.Conclusion: We found the abnormal high expression of KIF4A in human ccRCC tissues and demonstrated that KIF4A could serve as a tumor induction gene.Keywords: clear cell renal cell carcinoma, ccRCC, KIF4A, proliferation, prognosis, clinicopathological characteristics
