Development of a red fluorescent protein-based cGMP indicator applicable for live-cell imaging

Mai Takizawa; Yuri Osuga; Rika Ishida; Marie Mita; Kazuki Harada; Hiroshi Ueda; Tetsuya Kitaguchi; Takashi Tsuboi

doi:10.1038/s42003-022-03790-2

Communications Biology (Sep 2022)

Development of a red fluorescent protein-based cGMP indicator applicable for live-cell imaging

Mai Takizawa,
Yuri Osuga,
Rika Ishida,
Marie Mita,
Kazuki Harada,
Hiroshi Ueda,
Tetsuya Kitaguchi,
Takashi Tsuboi

Affiliations

Mai Takizawa: Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo
Yuri Osuga: Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo
Rika Ishida: Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo
Marie Mita: Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo
Kazuki Harada: Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo
Hiroshi Ueda: Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology
Tetsuya Kitaguchi: Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology
Takashi Tsuboi: Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo

DOI: https://doi.org/10.1038/s42003-022-03790-2
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 9

Abstract

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A red fluorescent protein-based indicator of cyclic guanosine 3′, 5′-monophosphate (cGMP) allows for multi-colour imaging and optogenetic applications while monitoring cGMP activity in cells.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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