Zoonotic Diseases (Feb 2024)

Occurrence of Picobirnavirus in Domestic and Wild Animals from Three Cities of Brazilian Amazon

  • Elaine Hellen Nunes Chagas,
  • Julia Rezende da Silva,
  • Bruno de Cássio Veloso de Barros,
  • José Wandilson Barbosa Duarte Júnior,
  • Fabiolla da Silva dos Santos,
  • Edivaldo Costa Sousa Júnior,
  • Delana Andreza Melo Bezerra,
  • Maria Inês dos Santos,
  • Helder Henrique Costa Pinheiro,
  • Yashpal Singh Malik,
  • Joana D’Arc Pereira Mascarenhas

DOI
https://doi.org/10.3390/zoonoticdis4010008
Journal volume & issue
Vol. 4, no. 1
pp. 74 – 85

Abstract

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This study aimed to detect picobirnavirus (PBV) in the fecal samples of wild and domestic animals from 2014 to 2016 in the Amazon biome. Fecal samples from different animals, including birds (n = 41) and mammals (n = 217), were used. The PAGE test showed negativity for PBV. However, 32 samples (12.4%, 32/258) showed positive results in RT-PCR analyses. Among the positive samples, pigs and cats, both with 28.12% (9/32), registered the highest frequencies. In a phylogenetic analysis, eight sequences from positive samples were grouped in the Genogroup 1 of PBV (PBV GI). PBV occurrence was significantly related to cats and pigs but not other mammals or birds, independently of their geographical origin. A nucleotide analysis demonstrated similarity among the feline group but the absence of a defined structure between the clades. PBVs are highly widespread viruses that can affect the most diverse types of hosts in the Amazon biome, including humans.

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