Upregulated Wnt-11 and miR-21 Expression Trigger Epithelial Mesenchymal Transition in Aggressive Prostate Cancer Cells
Elif Damla Arisan,
Ozge Rencuzogullari,
Ines Lua Freitas,
Syanas Radzali,
Buse Keskin,
Archana Kothari,
Antony Warford,
Pinar Uysal-Onganer
Affiliations
Elif Damla Arisan
Institute of Biotechnology, Gebze Technical University, Gebze 41400, Kocaeli, Turkey
Ozge Rencuzogullari
Department of Molecular Biology and Genetics, Istanbul Kultur University, Atakoy Campus, 34156 Istanbul, Turkey
Ines Lua Freitas
Cancer Research Group, School of Life Sciences, College of Liberal Arts and Sciences, University of Westminster, 115 New Cavendish Street, W1W 6UW, London, UK
Syanas Radzali
Cancer Research Group, School of Life Sciences, College of Liberal Arts and Sciences, University of Westminster, 115 New Cavendish Street, W1W 6UW, London, UK
Buse Keskin
Department of Molecular Biology and Genetics, Istanbul Kultur University, Atakoy Campus, 34156 Istanbul, Turkey
Archana Kothari
Department of Histopathology, Kingston Hospital, Galsworthy Road, KT2 7QE, London, UK
Antony Warford
Cancer Research Group, School of Life Sciences, College of Liberal Arts and Sciences, University of Westminster, 115 New Cavendish Street, W1W 6UW, London, UK
Pinar Uysal-Onganer
Cancer Research Group, School of Life Sciences, College of Liberal Arts and Sciences, University of Westminster, 115 New Cavendish Street, W1W 6UW, London, UK
: Prostate cancer (PCa) is the second-leading cause of cancer-related death among men. microRNAs have been identified as having potential roles in tumorigenesis. An oncomir, miR-21, is commonly highly upregulated in many cancers, including PCa, and showed correlation with the Wnt-signaling axis to increase invasion. Wnt-11 is a developmentally regulated gene and has been found to be upregulated in PCa, but its mechanism is unknown. The present study aimed to investigate the roles of miR-21 and Wnt-11 in PCa in vivo and in vitro. First, different Gleason score PCa tissue samples were used; both miR-21 and Wnt-11 expressions correlate with high Gleason scores in PCa patient tissues. This data then was confirmed with formalin-fixed paraffin cell blocks using PCa cell lines LNCaP and PC3. Cell survival and colony formation studies proved that miR-21 involves in cells’ behaviors, as well as the epithelial-mesenchymal transition. Consistent with the previous data, silencing miR-21 led to significant inhibition of cellular invasiveness. Overall, these results suggest that miR-21 plays a significant role related to Wnt-11 in the pathophysiology of PCa.
