Effect of Different Solvents on Antioxidant Activities and Secondery Metabolites of Haplophyllum suaveolens Varieties

Abstract

In this study, total bioactive contents and antioxidant activity levels of methanol and ethanol extracts of Haplophyllum suaveolens varieties were determined. Aerial parts of Haplophyllum suaveolens varieties which are Haplophyllum suaveolens (DC.) G. Don var. suaveolens (HS) and endemic Haplophyllum suaveolens var. cilicicum (Boiss.) C.C. Townsend (HC), were used in the extraction process. Total phenolic content was determined according to the Folin- Ciocalteu method and the amounts in each extract were expressed as mg gallic acid equivalent (GAE) g-1 extract. The total phenolic contents of the extracts varied between 39.54-44.16 mg GAE g-1 extract. Total flavonoid contents were measured as mg quercetin equivalent (QE) g-1 extract and varied between 17.30-41.40 mg QE g-1 extract. The methanol extracts of HS and HC exhibited maximum radical scavenging activities for DPPH radical (79.76% and 75.69%, respectively) and ABTS (54.74% and 55.13%, respectively). Thus, Haplophyllum suaveolens varieties exhibited strong radical scavenging activity and methanol extracts were found to be more effective than ethanol extracts in both varieties. In this study, it has been determined that Haplophyllum suaveolens varieties have strong radical scavenging activity and maybe good natural antioxidant sources for medical and commercial use.

Keywords

• antioxidant activity
• flavonoid
• haplophyllum l.
• phenolic