The intra-mitochondrial O-GlcNAcylation system rapidly modulates OXPHOS function and ROS release in the heart

Justine Dontaine; Asma Bouali; Frederic Daussin; Laurent Bultot; Didier Vertommen; Manon Martin; Raahulan Rathagirishnan; Alexanne Cuillerier; Sandrine Horman; Christophe Beauloye; Laurent Gatto; Benjamin Lauzier; Luc Bertrand; Yan Burelle

doi:10.1038/s42003-022-03282-3

Communications Biology (Apr 2022)

The intra-mitochondrial O-GlcNAcylation system rapidly modulates OXPHOS function and ROS release in the heart

Justine Dontaine,
Asma Bouali,
Frederic Daussin,
Laurent Bultot,
Didier Vertommen,
Manon Martin,
Raahulan Rathagirishnan,
Alexanne Cuillerier,
Sandrine Horman,
Christophe Beauloye,
Laurent Gatto,
Benjamin Lauzier,
Luc Bertrand,
Yan Burelle

Affiliations

Justine Dontaine: Pole of Cardiovascular Research (CARD), Institute of Experimental and Clinical Research (IREC), UCLouvain
Asma Bouali: Interdisciplinary School of Health Sciences, Faculty of Health Sciences, University of Ottawa
Frederic Daussin: Univ. Lille, Univ. Artois, Univ. Littoral Côte d’Opale, ULR 7369 - URePSSS - Unité de Recherche Pluridisciplinaire Sport Santé Société
Laurent Bultot: Pole of Cardiovascular Research (CARD), Institute of Experimental and Clinical Research (IREC), UCLouvain
Didier Vertommen: Pole of Protein phosphorylation (PHOS) and proteomic platform (MASSPROT), de Duve Institute (DDUV), UCLouvain
Manon Martin: Pole of Computational biology and bioinformatics (CBIO), de Duve Institute (DDUV), UCLouvain
Raahulan Rathagirishnan: Interdisciplinary School of Health Sciences, Faculty of Health Sciences, University of Ottawa
Alexanne Cuillerier: Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa
Sandrine Horman: Pole of Cardiovascular Research (CARD), Institute of Experimental and Clinical Research (IREC), UCLouvain
Christophe Beauloye: Pole of Cardiovascular Research (CARD), Institute of Experimental and Clinical Research (IREC), UCLouvain
Laurent Gatto: Pole of Computational biology and bioinformatics (CBIO), de Duve Institute (DDUV), UCLouvain
Benjamin Lauzier: Institute of Thorax, INSERM, CNRS, University of Nantes
Luc Bertrand: Pole of Cardiovascular Research (CARD), Institute of Experimental and Clinical Research (IREC), UCLouvain
Yan Burelle: Interdisciplinary School of Health Sciences, Faculty of Health Sciences, University of Ottawa

DOI: https://doi.org/10.1038/s42003-022-03282-3
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 14

Abstract

Read online

An in vitro assay in isolated heart mitochondria reveals that O-GlcNAcase inhibitor NButGT rapidly increases protein O-GlcNAcylation leading to increased respiratory capacity and complex I activity and decreased ROS release.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

About the journal